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NEDD4 (NM_198400) Human Untagged Clone

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SKU
SC123396
NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2
  $1,086.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NEDD4
Synonyms NEDD4-1; RPF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_198400 edited
ATGGCACAAAGCTTACGATTGCACTTTGCAGCCAGAAGAAGCAATACTTACCCTTTGTCA
GAAACCTCCGGAGATGACTTGGATAGCCATGTTCACATGTGCTTCAAAAGACCAACACGG
ATTTCAACGTCTAACGTTGTTCAAATGAAGCTGACTCCCAGACAGACTGCACTAGCTCCG
TTAATAAAGGAAAACGTTCAGTCTCAAGAAAGATCATCTGTTCCCTCATCTGAAAATGTT
AATAAAAAGAGCAGCTGTCTACAGATTTCACTACAGCCAACAAGGTACAGTGGATATCTT
CAGTCTAGCAATGTCTTAGCTGATAGTGATGATGCTTCGTTTACTTGTATCTTGAAGGAT
GGTATTTACAGTAGTGCTGTGGTCGATAATGAATTGAATGCTGTGAATGATGGTCACCTT
GTAAGCAGTCCAGCCATTTGTAGTGGTAGCCTTAGTAACTTTTCAACCAGTGATAATGGG
TCTTACAGCAGCAACGGTAGTGATTTTGGGTCATGTGCAAGTATCACAAGTGGAGGTTCA
TATACTAACAGTGTCATCAGTGACAGTAGTAGTTATACTTTTCCACCAAGTGATGATACT
TTTTTGGGTGGAAACTTACCTTCTGACAGCACCTCCAATAGAAGTGTGCCAAACAGGAAT
ACTACTCCTTGTGAAATTTTTTCAAGAAGTACAAGTACAGATCCTTTTGTCCAGGATGAC
TTGGAACATGGATTAGAGATTATGAAATTGCCAGTGAGCAGGAACACAAAAATTCCACTA
AAACGTTACTCCTCCTTAGTCATTTTTCCTAGGAGTCCTTCAACTACCCGACCGACTTCT
CCAACAAGTCTGTGTACTCTTCTGAGCAAAGGATCCTATCAAACTTCACACCAGTTTATT
ATTTCTCCTAGTGAAATTGCACATAATGAGGATGGCACTAGTGCTAAAGGATTTCTTTCA
ACAGCTGTCAATGGACTTCGGTTATCTAAAACAATTTGTACCCCCGGAGAAGTAAGAGAC
ATACGGCCGCTTCACAGGAAGGGCTCGTTACAGAAGAAAATTGTTCTTTCGAATAATACT
CCCAGACAGACTGTCTGTGAAAAGTCATCTGAAGGATATTCTTGTGTTTCAGTGCATTTC
ACCCAACGAAAAGCAGCTACATTAGACTGTGAAACAACAAATGGTGATTGTAAACCAGAA
ATGTCAGAAATTAAGCTTAATTCTGATTCAGAGTATATTAAGCTCATGCATAGGACATCT
GCATGTTTGCCATCCTCCCAAAATGTAGATTGTCAAATAAATATCAATGGAGAATTGGAA
AGACCACATTCACAGATGAACAAAAACCATGGTATTTTACGAAGAAGTATTTCATTGGGA
GGAGCTTATCCAAATATTTCCTGTCTATCCAGCCTTAAGCACAATTGTTCTAAAGGGGGA
CCATCTCAGTTACTCATAAAGTTTGCATCTGGAAATGAAGGTAAAGTGGATAATTTATCA
AGAGACAGCAACAGAGATTGCACAAATGAACTGTCTAATTCTTGCAAGCCTGGCTGGGTT
GTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACAAGAACCTTCTCCTCTA
CCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAA
TCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAAT
GGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGAA
ACAGAAAGTGTTGACAACCGAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAA
GCCACCATGTATAGCAACCAGGCCTTCCCATCACCTCCACCGTCAAGTAACTTGGATGTT
CCAACTCATCTTGCAGAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTG
AGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCTATACT
TTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCACCAGGT
TGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAACG
ACTACTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGC
CAGAGTTCTGCAGGCCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACC
CAGCCATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACCA
AATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACCTGGGAAGATCCAAGA
TTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATACTTCCAATGATCTAGGG
CCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTACATAAAT
CACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGA
CCAGCAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTTG
AAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTT
GAAGACTCTTACCGGAGAATTATGGGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACTG
TGGATTGAGTTTGATGGTGAAAAGGGATTGGATTATGGAGGAGTTGCCAGAGAATGGTTC
TTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACG
GACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACCTCTCT
TACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGAT
GGTTTTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGAT
ATGGAATCTGTGGATAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCA
ACAGAATTGGACCTCAGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACAT
GAGCTGAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAATATATT
TATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAAATGGCTGCTTTTAAA
GAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAATGAACTA
GAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAAG
TATAAAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTA
ATGATGGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTG
CCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAGTCATTTACAGTTGAA
CAGTGGGGTACTCCTGAAAAGCTGCCAAGAGCTCATACCTGTTTTAATCGCCTGGACTTG
CCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGATGGCAATTGAAAACACC
CAGGGCTTTGATGGAGTTGATTAG
Restriction Sites Please inquire |
ACCN NM_198400
Insert Size 5500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF was fully sequenced and matches to NM_198400.1.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198400.1, NP_940682.1
RefSeq Size 7084 bp
RefSeq ORF 3744 bp
Locus ID 4734
UniProt ID P46934
Cytogenetics 15q21.3
Protein Families Druggable Genome
Protein Pathways Endocytosis, Ubiquitin mediated proteolysis
Summary This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. provided by RefSeq, Jul 2016
Transcript Variant: This variant (2) encodes isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_198400" in other vectors (6)
SKU Description Size Price
RC212738 NEDD4 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 10 ug
$1,085.00
RC212738L1 Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged 10 ug
$1,385.00
RC212738L2 Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged 10 ug
$1,385.00
RC212738L3 Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged 10 ug
$1,385.00
RC212738L4 Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged 10 ug
$1,385.00
RG212738 NEDD4 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 10 ug
$1,285.00

file-alt Citations

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