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KAT5 (NM_006388) Human Over-expression Lysate

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SKU
LC401920
Lyophilized KAT5 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209059
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006388, NP_006379
Synonyms cPLA2; ESA1; HTATIP; HTATIP1; NEDFASB; PLIP; TIP; TIP60; ZC2HC5
Predicted MW 58.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006379
Locus ID 10524
Cytogenetics 11q13.1
Protein Families WB
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Other "KAT5" proteins (11)
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PH309059 KAT5 MS Standard C13 and N15-labeled recombinant protein (NP_006379) 10 ug
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LC405422 Lyophilized KAT5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405423 Lyophilized KAT5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY401920 Transient overexpression lysate of K(lysine) acetyltransferase 5 (KAT5), transcript variant 2 (as WB positive control) 100 ug
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LY405422 Transient overexpression lysate of K(lysine) acetyltransferase 5 (KAT5), transcript variant 3 (as WB positive control) 100 ug
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LY405423 Transient overexpression lysate of K(lysine) acetyltransferase 5 (KAT5), transcript variant 1 (as WB positive control) 100 ug
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TP309059 Recombinant protein of human K(lysine) acetyltransferase 5 (KAT5), transcript variant 2, 20 µg 20 ug
$867.00
TP310591 Recombinant protein of human K(lysine) acetyltransferase 5 (KAT5), transcript variant 3, 20 µg 20 ug
$867.00
TP315979 Recombinant protein of human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Elevation of androgen receptor promotes prostate cancer metastasis by induction of epithelial-mesenchymal transition and reduction of KAT5
Lin, Jan, Kuo et al
Cancer Sci. (2018) 109 (11), 3564-3574 DOI: 10.1111/cas.13776
AI Summary: OriGene’s TissueScan Prostate Tissue qPCR Array (HPRT101~103) was used to measure KAT5 mRNA levels across different stages of prostate cancer, helping to elucidate the relationship between KAT5 expression and prostate cancer progression in this study.
Snippet: The KAT5 mRNA level of human prostate tissue and different stages of prostate cancer was determined on TissueScan Prostate Tissue qPCR Array HPRT101~103 (OriGene Technologies, Rockville, MD, USA) according to the manufacturer's instructions with Maxima SYBR Green/ROX qPCR Master Mix (2×) (Fermentas, Glen Burnie, MA, USA) and analyzed by Applied Biosystems 7500 Real‐Time PCR system (Thermo Scientific, Waltham, MA, USA). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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