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Myd88 Mouse Gene Knockout Kit (CRISPR)

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SKU
KN310583
Myd88 - mouse gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

Frequently Bought Together (3)

MR227105 Myd88 (Myc-DDK-tagged) - Mouse myeloid differentiation primary response gene 88 (Myd88)
10 ug
$450.00
GE100003 pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
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TT300007S A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
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$265.00

Other products for "Myd88"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol Myd88
Locus ID 17874
Components

KN310583G1, Myd88 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTCTGCGGGAGACCCCCGCG

KN310583G2, Myd88 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCCACGTTAAGCGCGACCAA

KN310583D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

CAACGTTAAA GAGTAAGGTG GTAGGTTCCG CGTGTTTTTT GCAGACCCCG GGAAGGTAGT GCTGTTTGTC TCAGAAAACA AAGGACAAGA TACCCTAAAT GCCAATTTAT GGGCAAGGTC GTGGGCTTTC CCCTTTAAAA AGCAGAGTCA CAAGTGACAT CAGATAGCTA AAGGTAGACT GAGCCTTCGG GTTACTACTT CCGAAGCCAG TCTGTCTCTT CCCTCCCTAC TACCAGAATA TAAATTACTG TACAGAAGGG CTGTAACTCC TTCAAGTCCT AAACAAGGCT GATTGAGCTA ACCATGCATA ATAACCACTA CCAGATCGCC TAGTCCATCC ACCTTGATCG GTGGGCCAAG GCAGATGCCA GAGGCGGTTT ATATAAATTC TCACACCTTC GAGGGGAGGT GAGCAAACGT TAGAAGCACA AGTGGGTTGA CTTTTAGGCT GGGTAGTTCT GGCCCCGTAG TCTAGATTCT AGCAAAGGGC GGAGCTTCTC GGAAAGCGAA AGAAGGAGGG GCTGGCAGGA GACTTAAGGG AAGTAGGAAA CTCCACAGGC GAGCGTACTG GACGGCACCG GGGGCCCAGG GTTGCCTGCC ATCGCTGTTC TTGAACCCTC GGACGCCCGT GGCGGCCGAC TGGACCTTGC TGGCGGAGGA GATGGGCTTC GAGTACTTGG AGATCCGAGA GCTGGAAACG CGCCCTGACC CCACTCGCAG TTTGTTGGAT GCCTGGCAGG GGCGCTCTGG CGCGTCTGTC GGCAGGCTGC TAGAGCTGCT GGCCTTGTTA GACCGTGAGG ATATACTGAA GGAGCTGAAG TCGCGCATCG GTGAGGAAAC GTGCTTCCTG GCTTTGTGCC AAGGATCAAC GAGGCTAGCC TTGGCATATT AATCTTGTTC ATTTTTTTCT GGGGTGCGCT AGGGAGGAAG AGAGTGACAG TCGAATTTTC TGTCAGTATG CAGGTCAGCG TAGGGATTGG GAGAGGGGTC AAAAACCCTG AGGAAAGATG GAAAGAGCCT AGGCAAAGAC CTTCAGTTGT ACCAAGAGTC AAGATCTGCT TTTCTCGCGG TATCTCAGTT GTGTCAGGAT CAACCGGACA AGTGAGGACC CCTTCCATCA CTCACTGGCA CTCAGCACAA CACCCCCGCC CCGCCCACAG TGAGGTACAC CTTGCTAGAT GGGGGTCACC ATTCTCATTG

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_010851
UniProt ID P22366
Summary Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:9697844). Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9575168, PubMed:9697844). Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway (PubMed:9697844). Isoform 2 is defective in its ability to induce IRAK phosphorylation and NF-kappa-B activation and can function as a negative regulator of activation by IL-1 or lipopolysaccharide (LPS) (PubMed:11909531). Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes (PubMed:17018642). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (PubMed:17635956, PubMed:21998396). Mediates leukocyte recruitment at the inflammatory site (PubMed:18941239).UniProtKB/Swiss-Prot Function
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Documents
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Other Versions
SKU Description Size Price
GA202782 Myd88 CRISPRa kit - CRISPR gene activation of mouse myeloid differentiation primary response gene 88 1 kit
$1,657.00
KN310583BN Myd88 - mouse gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN310583LP Myd88 - mouse gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN310583RB Myd88 - mouse gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN510583 Myd88 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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