C1galt1c1 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN302341

C1galt1c1 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: C1galt1c1
• Locus ID: 59048
• Components:

  KN302341G1, C1galt1c1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCAGATGGTGATGCTCATGG

  KN302341G2, C1galt1c1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGATATCTCGAAAATTTCAG

  KN302341D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AAAAAGCCAA AAAATGAAAG AAAGAAAGAA AGAAAGAAAG AAAGAAAGAA AGAAAAGAAA GAAGGAAAGA AAGAAAAGAA AGGAAGAAAG AAAGAAAGGA AGGAAGAAAG AAAGAAAACG AAGAAAGAAA AGAGAAATTT GAGTTTAAAT TGAGTATCTG TTCAACTTAC AGGTCTGGAT GTAGAAGCTC TTTTATAATT AGCCAGAAGT ACTAACAGGG CAAGTTTGCC ATACAAAATA TTTAGAGAAC GATGGAAAAA CATTGTTTAT CACATTAGTT TCTAGGCTAA TGCTTTCACT TGCCACTTTG TTAAATAAAT GATGGATAAA ATTACTCCTC TACACATACA TACACACACA CACACACACA CACACACACA CTACAAATTC CAATCCTTGA ATGAGTTTCT ATAATGTTGC AGTTTCGACC CAATGCCAGT GGAAGGATAC TGAGTACTTG TCACAGTATG CTTCTCACCT GATTTTAAGC ATGTGGACCT TTGGTTTTTA AACATTAAGA ACAAATGTAA GTATATGAGA AATAACCAAG TAAAATTATT AGACTATGTA TTTGAAAAGT CTTTTTCTTT GCAGGAAAAA TTAGTCACCT ACTAGTTAGT CACGTGAGGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG CCTCTTTACG GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC TGCAGTACGT GATTCTTGAT CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC CTTGCGCTTA AGGAGCCCCT TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG GCCGCCGCGT GCGAATCTGG TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC TAGCCATTTA AAATTTTTGA TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG TAAATGCGGG CCAAGATCTG CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG GGGCCCGTGC GTCCCAGCGC ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA GAATCGGACG GGGGTAGTCT CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC CGTGTATCGC CCCGCCCTGG GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC GCTTCCCGGC CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC GGGTGAGTCA CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA GGCTGGAGAC GTGGAGGAGA ACCCTGGACC TATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATAATTGA TTAGTAGATA AGTGAAGCTC AGTAAGAGTT TCCGGGTATA CTGTATAGTT CTTGTAAAAC CCAAAGATGT GAGTCTTTGG GCTGCAGTGA AGGAGACTTG GACCAAACAC TGTGACAAAG CAGAATTCTT CAGTTCTGAA AATGTTAAAG TGTTTGAGTC AATTAATATG GACACAAATG ACATGTGGTT GATGATGAGG AAAGCTTACA AATATGCTTA TGATCAATAC AGGGACCAAT ACAACTGGTT CTTCCTTGCA CGCCCCACTA CTTTCGCTGT TATTGAAAAC CTCAAATATT TTTTGTTAAA AAAGGATCAA TCCCAACCTT TCTATCTCGG ACACACTGTA AAATCTGGAG ACCTTGAATA TGTGAGTGTG GATGGAGGAA TTGTCTTAAG CATAGAATCA ATGAAAAGAC TCAACAGTCT TCTCAGTGTT CCTGAAAAGT GTCCTGAACA AGGAGGAATG ATTTGGAAGA TATCTGAAGA TAAACAGCTG GCGGTCTGCC TGAAATACGC CGGAGTATTT GCAGAAAATG CCGAAGATGC CGATGGAAAA GATGTGTTTA ATACCAAATC CGTTGGCCTT TTCATTAAAG AGGCAATGAC TAACC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_021550
• UniProt ID: Q9JMG2
• Synonyms: 1500002I11Rik; AB030184; C1GalT2; C81205; Cosmc
• Summary: Probable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1) (By similarity).[UniProtKB/Swiss-Prot Function]