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IRE1 (ERN1) Human Gene Knockout Kit (CRISPR)

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SKU
KN215023
ERN1 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

Frequently Bought Together (3)

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Other products for "IRE1 or NM_001433"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol IRE1
Locus ID 2081
Components

KN215023G1, IRE1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CTCCCCGGTCACTCACCCCG

KN215023G2, IRE1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGGTGGAGGACAGCTCGCGA

KN215023D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

TTCGCTCCGG AGGTCGCCTC GGTAAACCTT GCGGATGATT CCACCACGGC GGAAAGACGA AAAGGAGGCT TCTAGGCACC CGGGACCGCC CGTGTCCCGC CCTTTTCCCC GATGAAGTCT GACGCGGCAG GTCCCTCGGC AGGGTTAATG GGACGGCCTG GTGGGGCATT GCCGGTCTTT CCCAGCCACA GACAAGCTTC GCGCCTCGAT CCCCCAGCGC CCCCAGACGC TGGATGCCGA GGCCTGGTCC TTTACAGCCG CCGACGCCTC ATGAGCGAAG CCAGCCAGAT GCCAGCACCG CCTTCGGCCC CGCCCCGAGC CAGCCCCTCC CACATCCCCG CCCGGGGCAC GCCCTCGGCC CGCCCCCATC TCCGCCCCGA ACACGCCCCT AGATTGGCCC CGCCCCGTCC CCGCCCGCAA CTCCGCCCCG ACCGGAAGAA GCCGCGGCAC CCAGCGCTTA TAGGGCCGGG AATGGGGGTG CCGCCGCTGC CTAGTCAGTT CTGCGTCCGC TGAGGCTCGG TCACCGCCTC GCTGTCGTCG CGGCGCCCCC GCCCCGTCCT CTGTCCGTAC CGCCCCCGGA GCCAGGGCCG AGTCCTCGCC CGGGGCAGCG TCGCGGCGCC GGGGCTGGAG TCCGAGCTGG GAGCGCGACG GCGGCGGCCA GATGCCCCGT GAGCCCAGCC GTGGGAGGCT CGGGCCGGGC GTAGGAGGCG GCACCGGGCC GGGAGAGCTT CCCTGACGGG AGTCGCGGCC CCACCGAGGC GTCGGCCGCC CGAAGAGCCT CCCAAGCCAG CGCGGCTGTC ACGGGCAGCG GCTCCCCGGA CCCCGCGGGC TCGGGCGCAA CTTCGTGGCG TCCTGCACGG CACTTGGGGG TCCCAGAAGG CCCCGGCCTG AGGAGGCCGC CCACCTGCGG GACCCTCCTC CTCGCGGGCG GGCGCCGGCG CTGCCTGGCG GGGGCCGCGG AGGAGCCGAA AGGGCGTTGG GATCCAGGGG AAGGGTCTGT CCTGCGCTTC GAATCCTTGT TTGAACTTTT TAAAAATTCG AAAACTTTGG GGAACGAATG CAACAGGGAG TAAGGACTCT CACCCGAGAG CTGGCTTCTG CCACTTGCCC TGCCGGTTTC TTGGTACTGG GAGGCTGCAG GCTTCCTTCT GTGCACCGAG ACTTCCTTGA GCAAGTGGAG CTGTGGGAAT TGCACTCTTG

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001433, NM_152461
UniProt ID O75460
Synonyms hIRE1p; IRE1; IRE1a; IRE1P
Summary This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. provided by RefSeq, Aug 2017
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Documents
Resources
Other Versions
SKU Description Size Price
GA101455 ERN1 CRISPRa kit - CRISPR gene activation of human endoplasmic reticulum to nucleus signaling 1 1 kit
$1,657.00
KN215023BN ERN1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN215023LP ERN1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN215023RB ERN1 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN415023 ERN1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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