RTEL1 Human Gene Knockout Kit (CRISPR)

CAT#: KN214507LP

RTEL1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: RTEL1
• Locus ID: 51750
• Components:

  KN214507G1, RTEL1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN214507G2, RTEL1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN214507LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  CTCCAGCCAG CCAGGAGGGC AGAGGGCTGG AGAGGCAGGG CCGGAGGTTC AGACCCTCCG CTCTGACGTT GCGCCTGGTG AGGCCGGGAG GGGTGCCGCT TGCCTCTTCA GCCCTCACGC TCTTGTGGAA GTCGCGGAAT TACTGCAGGC GGAACTTGCA GCACTGTGGG CGTCTTTTCC AGAGAAGGAC GGAGTTGTGG GGCGGGAGGA TAAGGCAAGG CCCAGCCACT TCGCATCTTC GCCCCGCCAG CTCCTCGAGA TGGGATATAC CAGGGTTGCT CTCCAACCCT CTCCGCAGGA GGGACTGATG GAAACGCCTG GGAAAGTAGC CCGGTACCCA CAAAGGCTGT CTACAAACAG AGTCTTACTG TCTTTCCCAG GTCTGTGCCA TAGGGATTCT CGAAGAGAAC AGCGTTGTGT CCCAGTGCAC ATGCTCGCAT CGCTTACCAG GAGTGCCCGA GACCCTAAGA TGTTCGGAGT GGTTTTTTCG CACAGACCCG AATAGCCTGC CCCTCAGCCA CGCTCTGTGC CCTTCTGAGA ACAGGCTGAT AGGTAGAGCA CAGGCCCCGA GGAAAGGACT GCGGGTGGGT GGAGCTTCAG CCAGGACGGG GTGTGCTTCC CTCTCCCGGC CCATTCCAGC CAGGCCCCTC CGGGCCAGAG GCAGCGTCTG TCATAAAAAG GGCTGGTGTT CCAGGTGGGG TCAGAGAGAG GATTGACAAG TAAAAACGAT CGTCCTTTGA AGGGGGCCGG CCCCTCCACA CCTGTGGGTA TTTCTCATCA GGCGGGACGA GAGACTGAGA AAATGAATAA GACACAGAGA CAAAGTATAG AGAGAAAAGT GGGCCCAGGG GACCGGCGCT CAGCATACAG AGGACCTGCA CCGGCACCAG TCTCTGAGTT TCCTCAGTAT TCATTAATTA CTATTTTCAC TATCTCAGCA AGAGGAATGC GGCAGGACAG CAAGGTGATA GTGGGGAGAA GGTCAGCAAG AAAACGTGAG CAAAGGAATC TGGGTCACAA ATAAGTTCAA GGGAAGGTAC TATGCCTGGA TGTGCACGTA GGCTAGTTTT ATGCTTTTCT CCACCCAAAC ATCTCGGTGG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001283009, NM_001283010, NM_016434, NM_032957
• UniProt ID: Q9NZ71
• Synonyms: C20orf41; DKCA4; DKCB5; NHL; RTEL
• Summary: This gene encodes a DNA helicase which functions in the stability, protection and elongation of telomeres and interacts with proteins in the shelterin complex known to protect telomeres during DNA replication. Mutations in this gene have been associated with dyskeratosis congenita and Hoyerall-Hreidarsson syndrome. Read-through transcription of this gene into the neighboring downstream gene, which encodes tumor necrosis factor receptor superfamily, member 6b, generates a non-coding transcript. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]