RTEL1 Rabbit Polyclonal Antibody

SKU TA336935

Rabbit Polyclonal RTEL1 Antibody

Product Data

• Application: ICC/IF, IP, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:250 - 1:500, Immunoprecipitation, Western Blot: 1:5000 - 1:10000
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A genomic peptide made to an internal region of the human RTEL1 protein (within residues 800-1000). [Swiss-Prot Q9NZ71]
• Buffer: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: regulator of telomere elongation helicase 1
• Database Link:
  NP_116575
  Q9NZ71
• Background: RTEL1 (Regulator of Telomere Length 1) is a nuclear iron-sulfur cluster containing ATP-dependent DNA helicase essential to genomic stability. RTEL1 contains 1 helicase ATP-binding domain and it belongs to helicase family, RAD3/XPD subfamily. Originally discovered in mouse, RTEL1 is a dominant factor controlling the regulation of telomeric length and genomic integrity, and recent evidence suggest that RTEL1 is preferentially recruited to, or exerts greater activity at genome's G-rich regions. In DNA double-strand breaks (DSBs) repair, RTEL1 disrupts D-loops in vitro and promotes synthesis-dependent strand annealing (SDSA) in vivo to direct DNA DSBs into non-crossover outcomes during mitotic repair. RTEL1 activity is also essential to meiotic recombination regulation as shown in C elegans. RTEL1's multiple roles in genomic stability maintenance and recombination regulation suggests it as a tumor suppressor. Indeed, RTEL1 mutation represents a risk factor for glioma and it is overexpressed in gastrointestinal cancers. Human RTEL1 deficiency caused Hoyeraal-Hreidarsson syndrome (HHS), a severe variant of Dyskeratosis Congenita (DC), characterized by early onset bone marrow failure, immunodeficiency and developmental defects.
• Synonyms: C20orf41; DKCA4; DKCB5; NHL; PFBMFT3; RTEL
• Note: In Immunohistochemistry and ICC/IF nuclear staining was observed. Formulin fixation is recommended for ICC/IF.

Reference Data

• Protein Families: Druggable Genome