PFKFB2 Human Gene Knockout Kit (CRISPR)

CAT#: KN211329LP

PFKFB2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: PFKFB2
• Locus ID: 5208
• Components:

  KN211329G1, PFKFB2 gRNA vector 1 in pCas-Guide CRISPR vector

  KN211329G2, PFKFB2 gRNA vector 2 in pCas-Guide CRISPR vector

  KN211329LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACGGTCT CTATCTCTTG ACCTCTTGAT CCGCCCGCCT CAGCCTCCCC AAGTGTTGGA ATTCCAGGAG TGAGCCACCG TGCCCGGCCT CACTGTTTGC AAAACTGAGT CCTACCGTTA GATTGGAGTT AATTTCCTGG TAATGTACTT CGTCTATACA TAACCCACCC CACCCCAACT GCTGCCACTA CTCTGCCCAG ATTCTTAAAA CTAGAATCTG AGGTCATGAA ACCTTGAGCA GGACACTTAA TTCGATGAAT CTCAAATTTT CAATCTGTAA AATGAGAAGA AATAATGGTG GCAAAATCTG CCTAATTACA AGATTATTGT TAGGACTGAA GAAGATAACG TCTGTAAATG TAGACTCGTA AGTCAGAAGA TAAATGTAAG GTTCTAGTGC AACTCTTTTT ATAGGTGAGG AAACAGAGGG CTAGAGAAGT AGCTGCTAAC TGGGAGAAAC AGGACTGAAC CTGCCTCTTG GCCCAGGGTG TTTTTACTTA TACCACACGG ATCCAATGCA CTGACTTTTT TTAAGTTATG TCTTCTTTCT TCCCCTTCCC TTTTTTACAT TCTACTTCTC TGTTTCAGAC ATCTGAAGAG CTGCCACTAG CATGGAAGAC GCCAAAAACA TAAAGAAAGG CCCAGCGCCA TTCTACCCAC TCGAAGACGG GACCGCCGGC GAGCAGCTGC ACAAAGCCAT GAAGCGCTAC GCCCTGGTGC CCGGCACCAT CGCCTTTACC GACGCACATA TCGAGGTGGA CATTACCTAC GCCGAGTACT TCGAGATGAG CGTTCGGCTG GCAGAAGCTA TGAAGCGCTA TGGGCTGAAT ACAAACCATC GGATCGTGGT GTGCAGCGAG AATAGCTTGC AGTTCTTCAT GCCCGTGTTG GGTGCCCTGT TCATCGGTGT GGCTGTGGCC CCAGCTAACG ACATCTACAA CGAGCGCGAG CTGCTGAACA GCATGGGCAT CAGCCAGCCC ACCGTCGTAT TCGTGAGCAA GAAAGGGCTG CAAAAGATCC TCAACGTGCA AAAGAAGCTA CCGATCATAC AAAAGATCAT CATCATGGAT AGCAAGACCG ACTACCAGGG CTTCCAAAGC ATGTACACCT TCGTGACTTC CCATTTGCCA CCCGGCTTCA ACGAGTACGA CTTCGTGCCC GAGAGCTTCG ACCGGGACAA AACCATCGCC CTGATCATGA ACAGTAGTGG CAGTACCGGA TTGCCCAAGG GCGTAGCCCT ACCGCACCGC ACCGCTTGTG TCCGATTCAG TCATGCCCGC GACCCCATCT TCGGCAACCA GATCATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TTCTTCGCTA AGAGCACTCT CATCGACAAG TACGACCTAA GCAACTTGCA CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA GGTGAGGCCG TGGCCAAACG CTTCCACCTA CCAGGCATCC GCCAGGGCTA CGGCCTGACA GAAACAACCA GCGCCATTCT GATCACCCCC GAAGGGGACG ACAAGCCTGG CGCAGTAGGC AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTGGACTTGG ACACCGGTAA GACACTGGGT GTGAACCAGC GCGGCGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC AACCCCGAGG CTACAAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAA TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGAGAGCA TCCTGCTGCA ACACCCCAAC ATCTTCGACG CCGGGGTCGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCCGCCGCA GTCGTCGTGC TGGAACACGG TAAAACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTTA CAACCGCCAA GAAGCTGCGC GGTGGTGTTG TGTTCGTGGA CGAGGTGCCT AAAGGACTGA CCGGCAAGTT GGACGCCCGC AAGATCCGCG AGATTCTCAT TAAGGCCAAG AAGGGCGGAA AGATCGCCGT GTAAGAATTC CGATCATATT CAATAACCCT TAATATAACT TCGTATAATG TATGCTATAC GAAGTTATTA GGTCTGAAGA GGAGTTTACG TCCAGCCAAG CTTAGGATCT CGACCTCGAA ATTCTACCGG GTAGGGGAGG CGCTTTTCCC AAGGCAGTCT GGAGCATGCG CTTTAGCAGC CCCGCTGGGC ACTTGGCGCT ACACAAGTGG CCTCTGGCCT CGCACACATT CCACATCCAC CGGTAGGCGC CAACCGACTC CGTTCTTTGG TGGCCCCTTC GCGCCACCTT CTACTCCTCC CCTAGTCAGG AAGTTCCCCC CCGCCCCGCA GCTCGCGTCG TGCAGGACGT GACAAATGGA AGTAGCACGT CTCACTAGTC TCGTGCAGAT GGACAGCACC GCTGAGCAAT GGAAGCGGGT AGGCCTTTGG GGCAGCGGCC AATAGCAGCT TTGCTCCTTC GCTTTCTGGG CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG CATTCTGCAC GCTTCAAAAG CGCACGTCTG CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC CTGCATCCAT CTAGATCTCG AGCAGCTGAA GCTTACCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCACAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGACGC CCGCCCCACG ACCCGCAGCG CCCGACCGAA AGGAGCGCAC GACCCCATGC ATCGATGATA TCAGATCCCC GGGATGCAGA AATTGATGAT CTATTAAACA ATAAAGATGT CCACTAAAAT GGAAGTTTTT CCTGTCATAC TTTGTTAAGA AGGGTGAGAA CAGAGTACCT ACATTTTGAA TGGAAGGATT GGAGCTACGG GGGTGGGGGT GGGGTGGGAT TAGATAAATG CCTGCTCTTT ACTGAAGGCT CTTTACTATT GCTTTATGAT AATGTTTCAT AGTTGGATAT CATAATTTAA ACAAGCAAAA CCAAATTAAG GGCCAGCTCA TTCCTCCCAC TCATGATCTA TAGATCTATA GATCTCTCGT GGGATCATTG TTTTTCTCTT GATTCCCACT TTGTGGTTCT AAGTACTGTG GTTTCCAAAT GTGTCAGTTT CATAGCCTGA AGAACGAGAT CAGCAGCCTC TGTTCCACAT ACACTTCATT CTCAGTATTG TTTTGCCAAG TTCTAATTCC ATCAGAAGCT GGTCGAGATC CGGAACCCTT AATATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCCCTCGAA GAGGTTCACT AGGCGCGCCC TCTCAGCATT TTATCTTGGG AATATAGTTA TAATCCTGGA CTCTGCTGCT TGGTGGGAGT GATAAATAAT TATTTGACAT GGAAATTTAA TGCAAAGTCT CCAGGGTAGA ATGTCCTGTC TAAAACAAGT AGACTTAGTG AAATGTCTGT TCTTGGGGCT TGACTACTGG TGTAGTCCAG CAGTTTCTTA CTGCTTTCTT AAACTGAATC TCTGTCTCCT GGGATGTTTG CACACTGGCT TGCTGATGGA AGTTAGAACT AACATTATTG TACACACAAT GAGTAAGGCA CAGTTGCAGG TGCAGGAATT ACTTGGAGCT GGTAATGTTA CCAGCTTTAT CTGTTGGGCT AAGTTTTGGG AGAGTGCTGC TCATTGTAGA AATTGCAGAT GGGAGCCAGT GCTGCTTGGT TTCCTTCCTA CAGGCCTGAG GGGAATGAGC CCTGACACTT GGCTTCTCAC AGTGTCTTGG GCATCTTACT TTCTCACAGG TTTAGCCTTT GTACTTTGTA AACTATTTTC CCTATAGGTT CATTTTTTTG AGGACAGCTT TCACATGAGC ATTTTCATTT CTATGCCTGT CTTTTAGATA GAAACAGCAT CACTCTCGCC GGTTGGACTT TAGATCAGAA GGGATCTTGC TGCCGCCCGA AAGAGGAAGG GCTGGAAGAG GAAGGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001018053, NM_006212
• UniProt ID: O60825
• Synonyms: PFK-2/FBPase-2
• Summary: The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]