ZNF281 Human Gene Knockout Kit (CRISPR)

CAT#: KN209243RB

ZNF281 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: ZNF281
• Locus ID: 23528
• Components:

  KN209243G1, ZNF281 gRNA vector 1 in pCas-Guide CRISPR vector

  KN209243G2, ZNF281 gRNA vector 2 in pCas-Guide CRISPR vector

  KN209243RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001281293, NM_001281294, NM_012482
• UniProt ID: Q9Y2X9
• Synonyms: ZBP-99; ZNP-99
• Summary: Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function]