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CD3E Human Gene Knockout Kit (CRISPR)

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SKU
KN208276
CD3E - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Other products for "CD3E or NM_000733"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol CD3E
Locus ID 916
Components

KN208276G1, CD3E gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTGGTGTGTCTCCAGACCGC

KN208276G2, CD3E gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATCCTACTCACCTGATAAG

KN208276D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

AAGATGGCTG CACCCTGCTG CTCCATGCCA GATCCACCAC ACAGAAAGCA AATGTTCAGT GCATCTCCCT CTTCCTGTCA GAGCTTATAG AGGAAGGAAG ACCCCGCAAT GTGGAGGCAT ATTGTATTAC AATTACTTTT AATGGCAAAA ACTGCAGTTA CTTTTGTGCC AACCTACTAC ATGGTCTGGA CAGCTAAATG TCATGTATTT TTCATGGCCC CTCCAGGTAT TGTCAGAGTC CTCTTGTTTG GCCTTCTAGG AAGGCTGTGG GACCCAGCTT TCTTCAACCA GTCCAGGTGG AGGCCTCTGC CTTGAACGTT TCCAAGTGAG GTAAAACCCG CAGGCCCAGA GGCCTCTCTA CTTCCTGTGT GGGGTTCAGA AACCCTCCTC CCCTCCCAGC CTCAGGTGCC TGCTTCAGAA AATGGTGAGT CTCTCTCTTA TAAAGCCCTC CTTTTTCATC CTAGCATTGG GAACAATGGC CCCAGGGTCC TTATCTCTAG CAGATGTTTT GAAAAAGTCA TCTGTTTTGC TTTTTTTCCA GAAGTAGTAA GTCTGCTGGC CTCCGCCATC TTAGTAAAGT AACAGTCCCA TGAAACAAAG ACAGCCTTAC CTGGCACTGC CTAGTGGCAC CAAGGAGCCT CATTTACCAG ATGTAAGGAA CTGTTTGTGC TATGTTAGGG TGAGGGATTA GAGCTGGGGA CTAAAGAAAA AGATAGGCCA CGGGTGCCTG GGAGAGCGTT CGGGGAGCAG GCAAAGAAGA GCAGTTGGGG TGATCATAGC TATTGTGAGC AGAGAGGTCT CGCTACCTCT AAGTACGAGC TCATTCCAAC TTACCCAGCC CTCCAGAACT AACCCAAAAG AGACTGGAAG AGCGAAGCTC CACTCCTTGT TTTGAAGAGA CCAGATACTT GCGTCCAAAC TCTGCACAGG GCATATATAG CAATTCACTA TCTTTGAGAC CATAAAACGC CTCGTAATTT TTAGTCCTTT TCAAGTGACC AACAACTTTC AGTTTATTTC ATTTTTTTGA AGCAAGATGG ATTATGAATT GATAAATAAC CAAGAGCATT TCTGTATCTC ATATGAGATA AATAATACCA AAAAAAGTTG CCATTTATTG TCAGATACTG TGTAAAGAAA AAATTATTTA GACGTGTTAA CTGGTTTAAT CCTACTTCTG CCTAGGAAGG AAGGTGTTAT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000733
UniProt ID P07766
Synonyms IMD18; T3E; TCRE
Summary The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. provided by RefSeq, Jul 2008
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Documents
Resources
Other Versions
SKU Description Size Price
GA100644 CD3E CRISPRa kit - CRISPR gene activation of human CD3e molecule 1 kit
$1,657.00
KN208276BN CD3E - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208276LP CD3E - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208276RB CD3E - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN408276 CD3E - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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