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PFKFB3 Human Gene Knockout Kit (CRISPR)

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SKU
KN206043
PFKFB3 - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

Frequently Bought Together (4)

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Other products for "PFKFB3 or NM_001145443"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol PFKFB3
Locus ID 5209
Components

KN206043G1, PFKFB3 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTGGACACGTTTAGTCCCA

KN206043G2, PFKFB3 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTGGCAGAGCCCCTGCTTGC

KN206043D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

CCTGCAGGGG CTGCCCTCCG CCTTTTCCGC CCTGCGTGCC AGAAAGGCCC GGCTACCTGG TGTTCCTACC CGCACCGGGG GGTCCCAGCG CACCGGACCC CGGTTTACAG GCCGGGTGGT TGGAGGCCCA GCACTGCCCA GAGCCCTGGG CCCCTCTCAG CTCAAAGACC CGGGAGAGAA GTGGGAGCTG CAGAAGTGTG GGGAGGGGGG CCTCGATTTG ACTTTCAAAT CAGAGTTGTG TGACTCCTGC GCCGTCTGTT TGGGTTGGGA GGGGTTGAGT TCTGGTGATG ATTGTCACTC AACACCCGAG ACTCCCCCAC TGCGACCTGG GCACACTAAG GACGTTGAGG ACTGGGGGTG TGTGTGGGGG GAGCCTGGCC TCCCCTGGCA CTGGGCCTGT GCTGTGCCGT CTCTCCCTTC CCGCACCCAC CCATCTGCCT CTCTTCTCTC ATAACTCAGA GGCACGGCCA GCGTGATGCT ACGGTTGCCT GGAGGCTGTA CCGGACTTGT TTTTCAAACA GAGGGTGCCT AGGTATCAGC GTGGGCTCCT GGCGGTGAAG GGGGTGGCTG CTGACTCTCC TGTCCCGTTG GGGTAGAGAG TCGGCCACCT TGACTCTGTG GGTTCTCTGG AGGCTCTCAG AGTGTCCCTC CCCCCCTACT CATTAACTGC AGGGGACAAT CATTTATCTC TGACTTCATC GCTTCTGCCC ACTTTTGTCG ATGTAGGCTC TGGTTGGGAA ACTCCTAAAA GGGACAGACT TCTGTTTACT TGTGCGCTCA GTAGAGAGCC TTATGCGGCA AAGTATTAAA TAGCCAAGCT GCTAATGTAT TGTTTGCATA TGAAGATTTT GCTTTATGAC AGCGTATTTT AGTGCATTAT TAGATCACAC AGGACAAAAC ATTTGCAAAC TGCCAACCAA GGTAAGCTTT GGTTTTTTGG TTATTATCAT TGTTTAATCT GAAGAGACCT TGCTGCATGA AGAGGAAAAT GTTATCCTTG CATTTGGCAG ATGCTACTTC CTGGAGGTCC CAGAGTGTAA GAAACTGTTG CTTGGCATAA GTGCTGTCTG CGTTTCCCGG TCACCCTGGT GTGAGCCAGG GCACAGGCTC TTGGAGGCTT TATCGAGGCA GAGCTTCCCC AGTTGCAGGT GGGACCTCTC ACTTAGAAGT AAGCACAGCA AGTGGGAACT GCAGGGAGCC

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001145443, NM_001282630, NM_001314063, NM_001323016, NM_001323017, NM_004566, NR_136554, NM_001363545
UniProt ID Q16875
Synonyms iPFK-2; IPFK2; PFK2
Summary The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2016
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Documents
Resources
Other Versions
SKU Description Size Price
GA103499 PFKFB3 CRISPRa kit - CRISPR gene activation of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 1 kit
$1,657.00
KN206043BN PFKFB3 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN206043LP PFKFB3 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN206043RB PFKFB3 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN406043 PFKFB3 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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