CYP1B1 Human Gene Knockout Kit (CRISPR)

CAT#: KN204074

CYP1B1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: CYP1B1
• Locus ID: 1545
• Components:

  KN204074G1, CYP1B1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TTAGCGGCCAAGGGTCGTTC

  KN204074G2, CYP1B1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCTGCTACTCCTGTCGGTGC

  KN204074D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AGGCACCCTT CTCCGTCCCC ATCCCAATCC AAGCGCTCCT GGCACTGACG ACGCCAAGAG ACTCGAGTGG GAGTTAAAGC TTCCAGTGAG GGCAGCAGGT GTCCAGGCCG GGCCTGCGGG TTCCTGTTGA CGTCTTGCCC TAGGCAAAGG TCCCAGTTCC TTCTCGGAGC CGGCTGTCCC GCGCCACTGG AAACCGCACC TCCCCGCAGG TCAGTCTGTC TGCCGAGGCG CTGCCCGGCG ACCTCTTCAG ATGGATTATT ACAGGTAGCG GGTGGCGTGG TAGGTACTTT AAAGGAAATC AAGCGCCACC GCCTCGATGC CCGCAGCGTT GTCCCCAGAT TGCAGGAACC GTTACGCGCC TTGCGGGGAG GGGAAGGGTT TGGCGCTGGG TTACAGCGAG GTGGAAACAC GCCCCTTCTC TTCTCCAAGG GAGAGTGGGT TGGGGATGGG AAGGGGCGTC TTCGGCCATT TCTCCAGAGA GTCAGCTCCG ACCTCTCCAC CCAACGGCAC TCAGTCCCCA GAGGCTGGGG TAGGGGCGTG GGGCGCCCGC TCCTGTCTCT GCACCCCTGA GTGTCACGCC TTCTCCTCTC TGTCCCCAGC TGGCCACTGT GCATGTGGGC CAGCGGCTGC TGAGGCAACG GAGGCGGCAG CTCCGGTCCG CGCCCCCGGG CCCGTTTGCG TGGCCACTGA TCGGAAACGC GGCGGCGGTG GGCCAGGCGG CTCACCTCTC GTTCGCTCGC CTGGCGCGGC GCTACGGCGA CGTTTTCCAG ATCCGCCTGG GCAGCTGCCC CATAGTGGTG CTGAATGGCG AGCGCGCCAT CCACCAGGCC CTGGTGCAGC AGGGCTCGGC CTTCGCCGAC CGGCCGGCCT TCGCCTCCTT CCGTGTGGTG TCCGGCGGCC GCAGCATGGC TTTCGGCCAC TACTCGGAGC ACTGGAAGGT GCAGCGGCGC GCAGCCCACA GCATGATGCG CAACTTCTTC ACGCGCCAGC CGCGCAGCCG CCAAGTCCTC GAGGGCCACG TGCTGAGCGA GGCGCGCGAG CTGGTGGCGC TGCTGGTGCG CGGCAGCGCG GACGGCGCCT TCCTCGACCC GAGGCCGCTG ACCGTCGTGG CCGTGGCCAA CGTCATGAGT GCCGTGTGTT TCGGCTGCCG CTACAGCCAC GACGACCCCG AGTTCCGTGA GCTGCTCAGC CACAACGAAG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000104
• UniProt ID: Q16678
• Synonyms: CP1B; CYPIB1; GLC3A; P4501B1
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]