PRMT5 Human Gene Knockout Kit (CRISPR)

SKU KN203458BN

PRMT5 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Vector: mBFP-Neo
• Target Symbol: PRMT5
• Locus ID: 10419
• Components:

  KN203458G1, PRMT5 gRNA vector 1 in pCas-Guide CRISPR vector

  KN203458G2, PRMT5 gRNA vector 2 in pCas-Guide CRISPR vector

  KN203458BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001039619, NM_001282953, NM_001282954, NM_001282955, NM_001282956, NM_006109
• UniProt ID: O14744
• Synonyms: HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs
• Summary: This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
• Shipping: Ambient