PRMT5 (NM_006109) Human Tagged ORF Clone
- TrueORF®
SKU
RC203458
PRMT5 (Myc-DDK-tagged)-Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1
$593.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PRMT5 |
| Synonyms | HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006109 |
| ORF Size | 1911 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006109.5 |
| RefSeq Size | 2541 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 10419 |
| UniProt ID | O14744 |
| Cytogenetics | 14q11.2 |
| Domains | Skb1 |
| Protein Families | Stem cell - Pluripotency |
| MW | 72.7 kDa |
| Summary | This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006109" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203458L1 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$893.00
|
|
| RC203458L2 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mGFP tagged | 10 ug |
$893.00
|
|
| RC203458L3 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$893.00
|
|
| RC203458L4 | Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mGFP tagged | 10 ug |
$893.00
|
|
| RG203458 | PRMT5 (tGFP-tagged) - Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1 | 10 ug |
$489.00
$793.00
|
|
| SC116333 | PRMT5 (untagged)-Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1 | 10 ug |
$776.00
|
|
Citations
Discovery of an ApoE4-targeted small-molecule SirT1 enhancer for the treatment of Alzheimer's disease
Campagna, Chandra, Teter et al
Sci Rep (2025) 15 (1), 14028 DOI: 10.1038/s41598-025-96131-2
Campagna, Chandra, Teter et al
Sci Rep (2025) 15 (1), 14028 DOI: 10.1038/s41598-025-96131-2
Cited by 5
AI Summary: In this study, OriGene products—including siRNA kit (#SR418510), siTran 2.0 reagent (#TT320001), pCMV6-Entry vector (#PS100001), and human ORF clones for PRMT1 (#RC224239), PRMT4 (#RC217483), PRMT8 (#RC205188), PRMT5 (#RC203458), and PRMT7 (#RC201672)—were used for gene knockdown and overexpression experiments to investigate the molecular mechanisms underlying SirT1 regulation in Alzheimer's disease models.
Snippet: #SR418510) or 100 nM scrambled siRNA (provided with kit Origene #SR418510) for 48 h using siTran 2.0 reagent following manufacturer's protocol (Origene # TT320001;lls/well were plated in a 24-well plate and cultured at 37 oC with 5% CO2 in DMEM supplemented with FBS and G418 and grown overnight. Cells were then transfected with 0.75 µg of pCMV6-Entry vector (Origene # PS100001) or the human ORF clone for PRMT1 (Origene # RC224239), PRMT4 (Origene # RC217483), PRMT8 (Origene # RC205188), PRMT5 (Origene # RC203458), or PRMT7 (Origene # RC201672) using Turbo
PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core
Lubyova, Hodek, Zabransky et al
PLoS ONE (2017) 12 (10), e0186982 DOI: 10.1371/journal.pone.0186982
Lubyova, Hodek, Zabransky et al
PLoS ONE (2017) 12 (10), e0186982 DOI: 10.1371/journal.pone.0186982
AI Summary: OriGene products contributed to this study by providing expression plasmids for PRMT1, PRMT3, PRMT5v1, PRMT5v2, PRMT7, PRMT9, and MEP50/WDR77, which were used to investigate the roles of these proteins in Hepatitis B virus replication and post-translational modification of the HBV core protein.
Snippet: In mammals, protein arginine methyltransferase 5, PRMT5, is the main type II enzyme responsible for the majority of symmetric dimethylarginine formation in polypeptides. Recent study reported that PRMT5 restricts Hepatitis B virus (HBV) replication through epigenetic Cached;;Page 1. RESEARCH ARTICLE PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core Barbora Lubyova1*, Jan Hodek1, Ales Zabransky1, Hana Prouzova1, Martin Hubalek1, Ivan Hirsch1,2, Jan Weber1* ... View as HTML;Expression plasmids for PRMT1, PRMT3, PRMT5v1 (splice variant 1), PRMT5v2 (splice variant 2), PRMT7, PRMT9 (Q6P2P2 in the UniProt Database, previously also referred to as PRMT10) and MEP50/WDR77 tagged with myc and Flag were purchased from OriGene Technologies
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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