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PRMT5 (NM_006109) Human Untagged Clone

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SKU
SC116333
PRMT5 (untagged)-Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1
  $776.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PRMT5
Synonyms HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_006109, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTCCAGCGGGAGGGACCTGAATTGCGTCC
CCGAAATAGCTGACACACTAGGGGCTGTGGCCAAGCAGGGGTTTGATTTCCTCTGCATGCCTGTCTTCCA
TCCGCGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGAC
CTACTGCTGTCAGGAAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACT
CAAAAGTGGAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGCATATTT
GGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGCCAGAGTTTTGACCAAC
CACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGTACCCTTGGTGGCACCAGAGGACCTGA
GAGATGATATAATTGAGAATGCACCAACTACACACACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGAT
GTGGTGGCACAACTTCCGGACTTTGTGTGACTATAGTAAGAGGATTGCAGTGGCTCTTGAAATTGGGGCT
GACCTCCCATCTAATCATGTCATTGATCGCTGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCCCCACTA
GCATTTTCCTGACCAATAAGAAGGGATTTCCTGTTCTTTCTAAGATGCACCAGAGGCTCATCTTCCGGCT
CCTCAAGTTGGAGGTGCAGTTCATCATCACAGGCACCAACCACCACTCAGAGAAGGAGTTCTGCTCCTAC
CTCCAATACCTGGAATACTTAAGCCAGAACCGTCCTCCACCTAATGCCTATGAACTCTTTGCCAAGGGCT
ATGAAGACTATCTGCAGTCCCCGCTTCAGCCACTGATGGACAATCTGGAATCTCAGACATATGAAGTGTT
TGAAAAGGACCCCATCAAATACTCTCAGTACCAGCAGGCCATCTATAAATGTCTGCTAGACCGAGTACCA
GAAGAGGAGAAGGATACCAATGTCCAGGTACTGATGGTGCTGGGAGCAGGACGGGGACCCCTGGTGAACG
CTTCCCTGCGGGCAGCCAAGCAGGCCGACCGGCGGATAAAGCTGTATGCTGTGGAGAAAAACCCAAATGC
CGTGGTGACGCTAGAGAACTGGCAGTTTGAAGAATGGGGAAGCCAAGTGACCGTAGTCTCATCAGACATG
AGGGAATGGGTGGCTCCAGAGAAAGCAGACATCATTGTCAGTGAGCTTCTGGGCTCATTTGCTGACAATG
AATTGTCGCCTGAGTGCCTGGATGGAGCCCAGCACTTCCTAAAAGATGATGGTGTGAGCATCCCCGGGGA
GTACACTTCCTTTCTGGCTCCCATCTCTTCCTCCAAGCTGTACAATGAGGTCCGAGCCTGTAGGGAGAAG
GACCGTGACCCTGAGGCCCAGTTTGAGATGCCTTATGTGGTACGGCTGCACAACTTCCACCAGCTCTCTG
CACCCCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATGATTGACAACAACCGCTATTGCAC
CTTGGAATTTCCTGTGGAGGTGAACACAGTACTACATGGCTTTGCCGGCTACTTTGAGACTGTGCTTTAT
CAGGACATCACTCTGAGTATCCGTCCAGAGACTCACTCTCCTGGGATGTTCTCATGGTTTCCCATCCTCT
TCCCTATTAAGCAGCCCATAACGGTACGTGAAGGCCAAACCATCTGTGTGCGTTTCTGGCGATGCAGCAA
TTCCAAGAAGGTGTGGTATGAGTGGGCTGTGACAGCACCAGTCTGTTCTGCTATTCATAACCCCACAGGC
CGCTCATATACCATTGGCCTCTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_006109 unedited
GTTCANAATTGTATACGACNACTATAGGCGGCNCGCGAATCGGCACCAGGCCTCGTGCCG
AATTCGGCACGAGGAGATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTC
CAGCGGGAGGGACCTGAATTGCGTCCCCGAAATAGCTGACACACTAGGGGCTGTGGCCAA
GCAGGGGTTTGATATCCTCTGCATGCCTGTCTTCCATCCGCGTTTCAAGAGGGAGTTCAT
TCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGACCTACTGCTGTCAGG
AAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAA
AGTGGAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGC
ATATTTGGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGC
CAGAGTTTTGACCAACCACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGT
ACCCTTGGTGGCACCAGAGGACCTGAGAGATGATATAATTGAGAATGCACCAACTACACA
CACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGATGTGGTGGCACAACTTCCGGACTTT
GTGTGACTATAGTAAGAGGATTGCAGTGGCTCTTGAAAATGGGCTGACCTCCCATCTAAT
CATGTCATTGATCGCTGGCTTGGGGAGCCCATCANAGCAGCCATTCTCCCCACTAGCATT
TTCCTGACCAAATAGAAGGNNATTCCTGNTNNCTTCTAGATGCACCAGAGGCTCATCTTT
CCGCTCCTCAGNTGGAGGTGCAGTTCATCATCACAGGCACAACCACACTCAGANAAGGAG
GTCTGCTCCTACCTCCATA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_006109 unedited
CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTGGCGGGCAGTAAAGGGCTATAACTTTATTTGTATTTCCTCTTACCCAAAACCCTTAA
AACAAGAACCGAAAAAGGGTGAAAATCCGTTCAAACCCCATGTTCTCAGGGATATTCCAG
GGAGTTCTTGAGGGTGAGTGCGTAACTTTAAATCCAGCACTAATTCCTCACCCCCTGGCC
TGAGGGCTTCATAAATTGGGGGCTTGAACCCTGCAATTAATTATAATCCCTTGCCCACCT
TGATGTAAGGCAGGAAAGCAAATTGAAATGCTCCTCTCTGATGGGCAAGGGGAATCACAG
CCCATAAATGTACTGCACCTTTTGTACTACAGGAACAAAACCTGAAGCTGCTTTCAAGGG
TCTGGACACTTGGCACGCAGGGCTAAAGGCCAATGGTATATGAACGGCCTGTGGGGTTAT
GAATAACAAAACAGACTGGTGCTTGTACAACCCACTCATACCACACCTTTTTGGAAATGC
TGGCTCGCCAAAAACCCCCACAGATGGGTTGGGCCTTACGTACCCGTATTGGCTGCTTAA
TAAGGAAGAAGATGGGAAACCATTAAAACCTTCCAGGAAAGTGAGTTTCTTGACGGCTAC
CTCCGATGGATGCCTCGATAAACCCAGTCCCAAGGTACCCCGGAAAAGCCTGTGTATCTG
GGTTACCTCCACAGAAATTCCAAGGCAAAACGGGGTGTCGCCATATAGGAACCTTGTGGG
TAGGCTGAAGGAAAACACGGCTGGGGTCAAAAACTTGTGGAAATTCGCACCCCACACCTT
AAGGGTCTTAAAAGGGCCCTAGGTACGGGGCTTCTCTACAGGTGGACTTATTGTAACTTG
GGGAAAACGGACCCCAAGAATGTCTCCCGGT
Restriction Sites NotI-NotI |
ACCN NM_006109
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006109.2, NP_006100.2
RefSeq Size 2541 bp
RefSeq ORF 2541 bp
Locus ID 10419
UniProt ID O14744
Cytogenetics 14q11.2
Domains Skb1
Protein Families Stem cell - Pluripotency
Summary This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Sep 2015
Transcript Variant: This variant (1) encodes the longest isoform (a).
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Documents
Resources
"NM_006109" in other vectors (6)
SKU Description Size Price
RC203458 PRMT5 (Myc-DDK-tagged)-Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1 10 ug
$593.00
RC203458L1 Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, Myc-DDK-tagged 10 ug
$893.00
RC203458L2 Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mGFP tagged 10 ug
$893.00
RC203458L3 Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, Myc-DDK-tagged 10 ug
$893.00
RC203458L4 Lenti ORF clone of Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1, mGFP tagged 10 ug
$893.00
RG203458 PRMT5 (tGFP-tagged) - Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1 10 ug
$489.00 $793.00

file-alt Citations

PRMT5- mediated symmetric arginine dimethylation is attenuated by mutant huntingtin and is impaired in Huntington's Disease (HD)
Ratovitski, Arbez, Stewart et al
Cell Cycle (2015)
Cited by 74
AI Summary: OriGene products contributed human PRMT5 cDNA and human MEP50 cDNA subcloned into pcDNA expression vectors, which were used for ectopic expression studies to investigate their roles in Huntington's Disease pathogenesis.
Snippet: HttN511–8Q/52Q constructs were produced from N511–82Q by random contractions of polyQ repeat in bacterial cells. HttN586–20Q and Htt-N586– 82Q plasmids were described previously.18 Human PRMT5 cDNA subcloned into pcDNA expression vector was obtained from Origene. …
Proliferative role of TRAF4 in breast cancer by upregulating PRMT5 nuclear expression
Yang, Wang, Ren et al
Tumour Biol. (2015)
Cited by 44
AI Summary: In this study, the PRMT5 plasmid (catalog number SC116333) from OriGene was used to investigate the role of PRMT5 in breast cancer cell proliferation and its regulatory interaction with TRAF4.
Snippet: purchased from Addgene. The plasmid for the Zn finger domain deletion mutant (Flag-ΔZn-TRAF4) was kindly provided by Dr. Bert W. O'Malley. The PRMT5 plas- mid (SC116333) was purchased from OriGene. The TRAF4, PRMT5 ... Cite SaveSaving
Showing 1-2 of 2 papers.
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