PRMT5 (NM_006109) Human Untagged Clone

SKU SC116333

PRMT5 (untagged)-Human protein arginine methyltransferase 5 (PRMT5), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PRMT5
• Synonyms: HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006109, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTCCAGCGGGAGGGACCTGAATTGCGTCC; CCGAAATAGCTGACACACTAGGGGCTGTGGCCAAGCAGGGGTTTGATTTCCTCTGCATGCCTGTCTTCCA; TCCGCGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGAC; CTACTGCTGTCAGGAAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACT; CAAAAGTGGAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGCATATTT; GGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGCCAGAGTTTTGACCAAC; CACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGTACCCTTGGTGGCACCAGAGGACCTGA; GAGATGATATAATTGAGAATGCACCAACTACACACACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGAT; GTGGTGGCACAACTTCCGGACTTTGTGTGACTATAGTAAGAGGATTGCAGTGGCTCTTGAAATTGGGGCT; GACCTCCCATCTAATCATGTCATTGATCGCTGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCCCCACTA; GCATTTTCCTGACCAATAAGAAGGGATTTCCTGTTCTTTCTAAGATGCACCAGAGGCTCATCTTCCGGCT; CCTCAAGTTGGAGGTGCAGTTCATCATCACAGGCACCAACCACCACTCAGAGAAGGAGTTCTGCTCCTAC; CTCCAATACCTGGAATACTTAAGCCAGAACCGTCCTCCACCTAATGCCTATGAACTCTTTGCCAAGGGCT; ATGAAGACTATCTGCAGTCCCCGCTTCAGCCACTGATGGACAATCTGGAATCTCAGACATATGAAGTGTT; TGAAAAGGACCCCATCAAATACTCTCAGTACCAGCAGGCCATCTATAAATGTCTGCTAGACCGAGTACCA; GAAGAGGAGAAGGATACCAATGTCCAGGTACTGATGGTGCTGGGAGCAGGACGGGGACCCCTGGTGAACG; CTTCCCTGCGGGCAGCCAAGCAGGCCGACCGGCGGATAAAGCTGTATGCTGTGGAGAAAAACCCAAATGC; CGTGGTGACGCTAGAGAACTGGCAGTTTGAAGAATGGGGAAGCCAAGTGACCGTAGTCTCATCAGACATG; AGGGAATGGGTGGCTCCAGAGAAAGCAGACATCATTGTCAGTGAGCTTCTGGGCTCATTTGCTGACAATG; AATTGTCGCCTGAGTGCCTGGATGGAGCCCAGCACTTCCTAAAAGATGATGGTGTGAGCATCCCCGGGGA; GTACACTTCCTTTCTGGCTCCCATCTCTTCCTCCAAGCTGTACAATGAGGTCCGAGCCTGTAGGGAGAAG; GACCGTGACCCTGAGGCCCAGTTTGAGATGCCTTATGTGGTACGGCTGCACAACTTCCACCAGCTCTCTG; CACCCCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATGATTGACAACAACCGCTATTGCAC; CTTGGAATTTCCTGTGGAGGTGAACACAGTACTACATGGCTTTGCCGGCTACTTTGAGACTGTGCTTTAT; CAGGACATCACTCTGAGTATCCGTCCAGAGACTCACTCTCCTGGGATGTTCTCATGGTTTCCCATCCTCT; TCCCTATTAAGCAGCCCATAACGGTACGTGAAGGCCAAACCATCTGTGTGCGTTTCTGGCGATGCAGCAA; TTCCAAGAAGGTGTGGTATGAGTGGGCTGTGACAGCACCAGTCTGTTCTGCTATTCATAACCCCACAGGC; CGCTCATATACCATTGGCCTCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006109 unedited; GTTCANAATTGTATACGACNACTATAGGCGGCNCGCGAATCGGCACCAGGCCTCGTGCCG; AATTCGGCACGAGGAGATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTC; CAGCGGGAGGGACCTGAATTGCGTCCCCGAAATAGCTGACACACTAGGGGCTGTGGCCAA; GCAGGGGTTTGATATCCTCTGCATGCCTGTCTTCCATCCGCGTTTCAAGAGGGAGTTCAT; TCAGGAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGACCTACTGCTGTCAGG; AAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAA; AGTGGAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGC; ATATTTGGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGC; CAGAGTTTTGACCAACCACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGT; ACCCTTGGTGGCACCAGAGGACCTGAGAGATGATATAATTGAGAATGCACCAACTACACA; CACAGAGGAGTACAGTGGGGAGGAGAAAACGTGGATGTGGTGGCACAACTTCCGGACTTT; GTGTGACTATAGTAAGAGGATTGCAGTGGCTCTTGAAAATGGGCTGACCTCCCATCTAAT; CATGTCATTGATCGCTGGCTTGGGGAGCCCATCANAGCAGCCATTCTCCCCACTAGCATT; TTCCTGACCAAATAGAAGGNNATTCCTGNTNNCTTCTAGATGCACCAGAGGCTCATCTTT; CCGCTCCTCAGNTGGAGGTGCAGTTCATCATCACAGGCACAACCACACTCAGANAAGGAG; GTCTGCTCCTACCTCCATA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006109 unedited; CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTGGCGGGCAGTAAAGGGCTATAACTTTATTTGTATTTCCTCTTACCCAAAACCCTTAA; AACAAGAACCGAAAAAGGGTGAAAATCCGTTCAAACCCCATGTTCTCAGGGATATTCCAG; GGAGTTCTTGAGGGTGAGTGCGTAACTTTAAATCCAGCACTAATTCCTCACCCCCTGGCC; TGAGGGCTTCATAAATTGGGGGCTTGAACCCTGCAATTAATTATAATCCCTTGCCCACCT; TGATGTAAGGCAGGAAAGCAAATTGAAATGCTCCTCTCTGATGGGCAAGGGGAATCACAG; CCCATAAATGTACTGCACCTTTTGTACTACAGGAACAAAACCTGAAGCTGCTTTCAAGGG; TCTGGACACTTGGCACGCAGGGCTAAAGGCCAATGGTATATGAACGGCCTGTGGGGTTAT; GAATAACAAAACAGACTGGTGCTTGTACAACCCACTCATACCACACCTTTTTGGAAATGC; TGGCTCGCCAAAAACCCCCACAGATGGGTTGGGCCTTACGTACCCGTATTGGCTGCTTAA; TAAGGAAGAAGATGGGAAACCATTAAAACCTTCCAGGAAAGTGAGTTTCTTGACGGCTAC; CTCCGATGGATGCCTCGATAAACCCAGTCCCAAGGTACCCCGGAAAAGCCTGTGTATCTG; GGTTACCTCCACAGAAATTCCAAGGCAAAACGGGGTGTCGCCATATAGGAACCTTGTGGG; TAGGCTGAAGGAAAACACGGCTGGGGTCAAAAACTTGTGGAAATTCGCACCCCACACCTT; AAGGGTCTTAAAAGGGCCCTAGGTACGGGGCTTCTCTACAGGTGGACTTATTGTAACTTG; GGGAAAACGGACCCCAAGAATGTCTCCCGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_006109
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006109.2, NP_006100.2
• RefSeq Size: 2541 bp
• RefSeq ORF: 2541 bp
• Locus ID: 10419
• UniProt ID: O14744
• Cytogenetics: 14q11.2
• Domains: Skb1
• Protein Families: Stem cell - Pluripotency
• Summary: This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]; Transcript Variant: This variant (1) encodes the longest isoform (a).