Cyclophilin A (PPIA) Human Gene Knockout Kit (CRISPR)

SKU KN203307BN

PPIA - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Vector: mBFP-Neo
• Target Symbol: Cyclophilin A
• Locus ID: 5478
• Components:

  KN203307G1, Cyclophilin A gRNA vector 1 in pCas-Guide CRISPR vector

  KN203307G2, Cyclophilin A gRNA vector 2 in pCas-Guide CRISPR vector

  KN203307BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001300981, NM_021130, NM_203430, NM_203431
• UniProt ID: P62937
• Synonyms: CYPA; CYPH; HEL-S-69p
• Summary: This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]
• Shipping: Ambient