Cyclophilin A (PPIA) (NM_021130) Human Tagged ORF Clone
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Cyclophilin A |
| Synonyms | CYPA; CYPH; HEL-S-69p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_021130 |
| ORF Size | 495 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_021130.5 |
| RefSeq Size | 2288 bp |
| RefSeq ORF | 498 bp |
| Locus ID | 5478 |
| UniProt ID | P62937 |
| Cytogenetics | 7p13 |
| Domains | pro_isomerase |
| MW | 18 kDa |
| Summary | This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_021130" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203307L1 | Lenti ORF clone of Human peptidylprolyl isomerase A (cyclophilin A) (PPIA), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC203307L2 | Lenti ORF clone of Human peptidylprolyl isomerase A (cyclophilin A) (PPIA), mGFP tagged | 10 ug |
$525.00
|
|
| RC203307L3 | Lenti ORF clone of Human peptidylprolyl isomerase A (cyclophilin A) (PPIA), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC203307L4 | Lenti ORF clone of Human peptidylprolyl isomerase A (cyclophilin A) (PPIA), mGFP tagged | 10 ug |
$525.00
|
|
| RG203307 | PPIA (tGFP-tagged) - Human peptidylprolyl isomerase A (cyclophilin A) (PPIA) | 10 ug |
$489.00
|
|
| SC111937 | PPIA (untagged)-Human peptidylprolyl isomerase A (cyclophilin A) (PPIA) | 10 ug |
$225.00
|
|
Citations
Effect of internal cleavage site mutations in human immunodeficiency virus type 1 capsid protein on its structure and function
Tóth, Kádas, Mótyán et al
FEBS Open Bio (2016) 6 (8), 847-59 DOI: 10.1002/2211-5463.12094
Tóth, Kádas, Mótyán et al
FEBS Open Bio (2016) 6 (8), 847-59 DOI: 10.1002/2211-5463.12094
Cited by 3
AI Summary: In this study, OriGene products—including the human HIV-1 capsid protein cDNA clone (Cat# RC210125) and anti-cyclophilin A antibody (Cat# TA500011)—were used to generate mutant capsid proteins and to assess their structure and cyclophilin A-binding, respectively, thereby contributing to the characterization of how internal cleavage site mutations affect HIV-1 capsid protein function.
Proline isomerisation as a novel regulatory mechanism for p38MAPK activation and functions
Brichkina, Nguyen, Baskar et al
Cell Death Differ. (2016)
Brichkina, Nguyen, Baskar et al
Cell Death Differ. (2016)
Cited by 24
AI Summary: In this study, OriGene products—specifically Flag-tagged cyclophilin A, cyclophilin B, cyclophilin H, and CWC27—were used to investigate how different cyclophilins regulate p38MAPK activation through proline isomerisation.
Snippet: potent clones were used for further studies. Flag-tagged cyclophilin A, cyclophilin B, cyclophilin H and CWC27 were purchased from Origene (Rockville, MD, USA) (in pCMV-entry vector). Full-length human p38MAPK was cloned ... Related articles All 4 versions Cite SaveSaving
Cyclophilin B enhances HIV-1 infection
DeBoer, Madson, Belshan
Virology (2016) 489, 282-91 DOI: 10.1016/j.virol.2015.12.015
DeBoer, Madson, Belshan
Virology (2016) 489, 282-91 DOI: 10.1016/j.virol.2015.12.015
AI Summary: OriGene products contributed to this study by providing the C-terminal FLAG-tagged CypB and CypA ORFs, which were essential for generating expression constructs used to investigate the role of Cyclophilin B in enhancing HIV-1 infection.
Snippet: The C-terminal FLAG-tagged CypB and CypA ORFs were obtained from Origene Technologies (Rockville, MD). The CypB ORF was moved by PCR into the pTarget eukaryotic expression vector (Promega Corp., Madison WI USA), the backbone used for the deletion mutant ORFs which were constructed and inserted by PCR mutagenesis
Showing 1-3 of 3 papers.
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