Integrin beta 4 binding protein (EIF6) Human Gene Knockout Kit (CRISPR)

SKU KN200568RB

EIF6 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Vector: RFP-BSD
• Target Symbol: Integrin beta 4 binding protein
• Locus ID: 3692
• Components:

  KN200568G1, Integrin beta 4 binding protein gRNA vector 1 in pCas-Guide CRISPR vector

  KN200568G2, Integrin beta 4 binding protein gRNA vector 2 in pCas-Guide CRISPR vector

  KN200568RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001267810, NM_002212, NM_181466, NM_181467, NM_181468, NM_181469, NR_052022, NR_052023, NR_052024
• UniProt ID: P56537
• Synonyms: b(2)gcn; CAB; eIF-6; EIF3A; ITGB4BP; p27(BBP); p27BBP
• Summary: Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
• Shipping: Ambient