Syndecan 1 (SDC1) Human Gene Knockout Kit (CRISPR)

SKU KN200419RB

SDC1 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Vector: RFP-BSD
• Target Symbol: Syndecan 1
• Locus ID: 6382
• Components:

  KN200419G1, Syndecan 1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200419G2, Syndecan 1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200419RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001006946, NM_002997
• UniProt ID: P18827
• Synonyms: CD138; SDC; SYND1; syndecan
• Summary: The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
• Shipping: Ambient