IGF1 Receptor (IGF1R) (NM_000875) Human Tagged ORF Clone
$1,754.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | IGF1 Receptor |
| Synonyms | CD221; IGFIR; IGFR; JTK13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000875 |
| ORF Size | 4101 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000875.5 |
| RefSeq Size | 4989 bp |
| RefSeq ORF | 4104 bp |
| Locus ID | 3480 |
| UniProt ID | P08069 |
| Cytogenetics | 15q26.3 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Adherens junction, Colorectal cancer, Endocytosis, Focal adhesion, Glioma, Long-term depression, Melanoma, Oocyte meiosis, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer |
| MW | 154.79 kDa |
| Summary | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, May 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000875" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214928L1 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), Myc-DDK-tagged | 10 ug |
$2,054.00
|
|
| RC214928L2 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), mGFP tagged | 10 ug |
$2,054.00
|
|
| RC214928L3 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), Myc-DDK-tagged | 10 ug |
$2,054.00
|
|
| RC214928L4 | Lenti ORF clone of Human insulin-like growth factor 1 receptor (IGF1R), mGFP tagged | 10 ug |
$2,054.00
|
|
| RC600009 | IGF1R (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens insulin-like growth factor 1 receptor, Signal peptide (1-30) plus EC domain (741-935) | 10 ug |
$589.00
$1,170.00
|
|
| RG214928 | IGF1R (tGFP-tagged) - Human insulin-like growth factor 1 receptor (IGF1R) | 10 ug |
$789.00
$1,954.00
|
|
| SC124152 | IGF1R (untagged)-Human insulin-like growth factor 1 receptor (IGF1R) | 10 ug |
$1,755.00
|
|
Citations
Genotype-Structure-Phenotype Correlations in Disease-Associated IGF1R Variants and Similarities to Those in INSR Variants
Hosoe, Kawashima Sonoyama, Miya et al
Diabetes (2021)
Hosoe, Kawashima Sonoyama, Miya et al
Diabetes (2021)
Cited by 4
AI Summary: OriGene’s DDK tagged-pCMV-human IGF1R cDNA was used to construct wild-type and mutant IGF1R expression vectors, enabling the functional analysis of disease-associated IGF1R variants in this study.
Snippet: the approved guidelines. Plasmid construction DDK tagged-pCMV-human IGF1R cDNA (Origene, Rockville, MD) was used. We constructed mutant IGF1R expression vectors with the variant we previously reported [c.2216G
miR‑589‑3p sponged by the lncRNA TINCR inhibits the proliferation, migration and invasion and promotes the apoptosis of breast cancer cells by suppressing the Akt pathway via IGF1R
Guo, Zhu, Zhao et al
Int. J. Mol. Med. (2020) 46 (3), 989-1002 DOI: 10.3892/ijmm.2020.4666
Guo, Zhu, Zhao et al
Int. J. Mol. Med. (2020) 46 (3), 989-1002 DOI: 10.3892/ijmm.2020.4666
Cited by 34
AI Summary: In this study, OriGene's TINCR small-interfering RNA (siTINCR; cat. no. SR316884) and negative control siRNA (cat. no. SR30004) were used to modulate TINCR expression in breast cancer cells, enabling the investigation of TINCR's role in regulating cell proliferation, migration, invasion, and apoptosis.
The crosstalk between IGF-1R and ER-α in the proliferation and anti-inflammation of nucleus pulposus cells
Chen, Zhang, Zhu et al
Eur Rev Med Pharmacol Sci (2020) 24 (11), 5886-5894 DOI: 10.26355/eurrev_202006_21481
Chen, Zhang, Zhu et al
Eur Rev Med Pharmacol Sci (2020) 24 (11), 5886-5894 DOI: 10.26355/eurrev_202006_21481
Cited by 11
AI Summary: OriGene products, specifically the human tagged ORF clones for IGF-1R (NM_000875), ER-α (NM_000125), and the untagged clone (null, AK098156), were used for plasmid transfection to upregulate IGF-1R and ER-α expression in nucleus pulposus cells, enabling the study of their crosstalk in cell proliferation and inflammation.
Snippet: Plasmid Transfection NP cells were transfected by plasmid (hu- man tagged ORF clone) targeting IGF-1R (NM_000875), ER-α (NM_000125) or Untagged Clone (null, AK098156) purchased from OriGene Technologies (Rockville, MD, USA) using Turbo- Fectin regent;- Plasmid Transfection NP cells were transfected by plasmid (hu- man tagged ORF clone) targeting IGF-1R (NM_000875), ER-α (NM_000125) or Untagged Clone (null, AK098156) purchased from OriGene Technologies (Rockville, MD, USA) using Turbo- Fectin regent
Let-7c inhibits migration and epithelial-mesenchymal transition in head and neck squamous cell carcinoma by targetingIGF1RandHMGA2
Hou, Ishinaga, Midorikawa et al
Oncotarget (2018) 9 (10), 8927-8940 DOI: 10.18632/oncotarget.23826
Hou, Ishinaga, Midorikawa et al
Oncotarget (2018) 9 (10), 8927-8940 DOI: 10.18632/oncotarget.23826
Snippet: miRNA plasmid transfection. The let-7c expression vector pCMV-MIR-let-7c and the pCMV-MIR vector were obtained from Origene (Rockville, MD, USA) Three vectors (Origene), namely, pCMV6-control, pCMV6-IGF1R, and pCMV6-HMGA2, were used for the rescue
Anti-IGF-1R monoclonal antibody inhibits the carcinogenicity activity of acquired trastuzumab-resistant SKOV3
Wang, Zhang, Lv et al
J Ovarian Res (2014) 7 (1), 103 DOI: 10.1186/s13048-014-0103-5
Wang, Zhang, Lv et al
J Ovarian Res (2014) 7 (1), 103 DOI: 10.1186/s13048-014-0103-5
Cited by 7
AI Summary: In this study, the pCMV6-IGF1R plasmid from OriGene was used to manipulate IGF-1R expression, enabling investigation of its role in trastuzumab-resistant ovarian cancer cells.
Snippet: from Pierce;Cell culture medium Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from HyClone;Human IGF-1, NRG1-β1/HRG1-β1 was from R &D;IGF-1R expressing plasmid pCMV6-IGF1R was from OriGene;Lentiviral ... Cite SaveSaving
miR-195 inhibits the growth and metastasis of NSCLC cells by targeting IGF1R
Wang, Wang, Lan et al
Tumour Biol. (2014)
Wang, Wang, Lan et al
Tumour Biol. (2014)
Cited by 43
Showing 1-6 of 6 papers.
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