BMP2 (NM_001200) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BMP2 |
| Synonyms | BDA2; BMP2A; SSFSC; SSFSC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001200 |
| ORF Size | 1188 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001200.4 |
| RefSeq Size | 1547 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 650 |
| UniProt ID | P12643 |
| Cytogenetics | 20p12.3 |
| Domains | TGF-beta, TGFb_propeptide |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane |
| Protein Pathways | Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway |
| MW | 44.63 kDa |
| Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. provided by RefSeq, Jul 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001200" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214586L1 | Lenti ORF clone of Human bone morphogenetic protein 2 (BMP2), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC214586L2 | Lenti ORF clone of Human bone morphogenetic protein 2 (BMP2), mGFP tagged | 10 ug |
$757.00
|
|
| RC214586L3 | Lenti ORF clone of Human bone morphogenetic protein 2 (BMP2), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC214586L4 | Lenti ORF clone of Human bone morphogenetic protein 2 (BMP2), mGFP tagged | 10 ug |
$757.00
|
|
| RG214586 | BMP2 (tGFP-tagged) - Human bone morphogenetic protein 2 (BMP2) | 10 ug |
$489.00
$657.00
|
|
| SC119392 | BMP2 (untagged)-Human bone morphogenetic protein 2 (BMP2) | 10 ug |
$457.00
|
|
Citations
Cell-permeable bone morphogenetic protein 2 facilitates bone regeneration by promoting osteogenesis
Kang, Lee, Seo et al
Materials Today Bio (2024) 25, 100983 DOI: 10.1016/j.mtbio.2024.100983
Kang, Lee, Seo et al
Materials Today Bio (2024) 25, 100983 DOI: 10.1016/j.mtbio.2024.100983
Cited by 11
AI Summary: OriGene contributed the full-length human BMP2 cDNA (product: RC214586), which was used to construct expression vectors for generating the modified, cell-permeable BMP2 proteins evaluated in this study.
Snippet: on for the treatment of bone defects. 2 Materials and methods 2.1 Construction of E. coli expression vectors for the recombinant BMP The full-length cDNA for human BMP2 (RC214586) was purchased from Origene (Rockville, MD). To develop cell- and tissue-permeable recombinant BMP proteins, expression vectors were designed by fusing the DNA sequence of mature form (aa 282–396) encoding the human BM;The full-length cDNA for human BMP2 (RC214586) was purchased from Origene (Rockville, MD). To develop cell- and tissue-permeable recombinant BMP proteins, expression vectors were designed by fusing the DNA sequence of mature form (aa 282–396) encoding the human BMP2 gene with an aMTD and/or solubilization domain (SD),
Novel Synthesized Nanofibrous Scaffold Efficiently Delivered hBMP-2 Encoded in Adenoviral Vector to Promote Bone Regeneration
Zhu, Li, Zhang et al
J Biomed Nanotechnol (2017) 13 (4), 437-46 DOI: 10.1166/jbn.2017.2361
Zhu, Li, Zhang et al
J Biomed Nanotechnol (2017) 13 (4), 437-46 DOI: 10.1166/jbn.2017.2361
Cited by 26
AI Summary: In this paper, OriGene’s AdCMV-hBMP2 adenoviral vector was used to encode and deliver the hBMP-2 gene via a novel nanofibrous scaffold, enabling efficient gene transduction and promoting bone regeneration in vitro and in vivo.
Delivery of rhBMP-2 Plasmid DNA Complexes via a PLLA/Collagen Electrospun Scaffold Induces Ectopic Bone Formation
Zhao, Komatsu, Hadjiargyrou
J Biomed Nanotechnol (2016) 12 (6), 1285-96 DOI: 10.1166/jbn.2016.2250
Zhao, Komatsu, Hadjiargyrou
J Biomed Nanotechnol (2016) 12 (6), 1285-96 DOI: 10.1166/jbn.2016.2250
Cited by 35
AI Summary: In this study, OriGene’s human BMP-2 plasmid DNA (Cat# SC119688) was used to create DNA complexes adsorbed onto electrospun PLLA/Collagen I scaffolds, enabling the delivery and expression of rhBMP-2 for evaluating bone formation in vitro and in vivo.
Bioreducible-Cationic Poly(amido amine)s for Enhanced Gene Delivery and Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells
Jeong, Lee, Jung et al
J Biomed Nanotechnol (2016) 12 (5), 1023-34 DOI: 10.1166/jbn.2016.2223
Jeong, Lee, Jung et al
J Biomed Nanotechnol (2016) 12 (5), 1023-34 DOI: 10.1166/jbn.2016.2223
Cited by 15
AI Summary: OriGene’s human BMP-2 plasmid (Cat# SC120176) was used as the genetic material for transfection experiments, enabling the evaluation of the novel gene delivery system’s efficiency and its effect on osteogenic differentiation in tonsil-derived mesenchymal stem cells.
Nanoplex-Mediated Codelivery of Fibroblast Growth Factor and Bone Morphogenetic Protein Genes Promotes Osteogenesis in Human Adipocyte-Derived Mesenchymal Stem Cells
Atluri, Seabold, Hong et al
Mol. Pharm. (2015) 12 (8), 3032-42 DOI: 10.1021/acs.molpharmaceut.5b00297
Atluri, Seabold, Hong et al
Mol. Pharm. (2015) 12 (8), 3032-42 DOI: 10.1021/acs.molpharmaceut.5b00297
Cited by 48
AI Summary: OriGene products contributed plasmid DNA encoding FGF-2 (RC217445) and BMP-2 (RC213418), which were used to transfect human adipose-derived mesenchymal stem cells and study their combined effect on promoting osteogenesis in this research.
Snippet: Plasmid DNA (4.9 Kb) encoding basic fibroblast growth factor 2 protein (FGF-2) driven by CMV promoter/enhancer was obtained from Origene Technologies, Inc. (Rockville, MD). Plasmid DNA (5.0 Kb) encoding bone morphogenetic protein 2 protein (BMP-2) driven by CMV promoter/enhancer was obtained from Origene Technologies, Inc
Inactivation of bone morphogenetic protein 2 may predict clinical outcome and poor overall survival for renal cell carcinoma through epigenetic pathways
Mitsui, Hirata, Arichi et al
Oncotarget (2015) 6 (11), 9577-9591
Mitsui, Hirata, Arichi et al
Oncotarget (2015) 6 (11), 9577-9591
Snippet: For BMP-2 overexpression, Caki-1 and Caki-2 cells were transfected with a pCMV6-ENTRY vector expressing human BMP-2 cDNA or an empty pCMV6- ENTRY vector (OriGene Technologies, Rockville, MD) using X-treme gene HD Transfection Reagent (Roche ... Cite SaveSaving
Showing 1-6 of 8 papers.
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