Her2 (ERBB2) (NM_004448) Human Tagged ORF Clone
SKU
RC212583
ERBB2 (Myc-DDK-tagged)-Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1
$1,092.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Her2 |
| Synonyms | CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004448 |
| ORF Size | 3765 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004448.4 |
| RefSeq Size | 4624 bp |
| RefSeq ORF | 3768 bp |
| Locus ID | 2064 |
| UniProt ID | P04626, X5DNK3 |
| Cytogenetics | 17q12 |
| Domains | FU, Furin-like, pkinase, Recep_L_domain, S_TKc, TyrKc, YLP |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer |
| MW | 137.7 kDa |
| Summary | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004448" in other vectors (9)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212583L1 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,392.00
|
|
| RC212583L2 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, mGFP tagged | 10 ug |
$1,392.00
|
|
| RC212583L3 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,392.00
|
|
| RC212583L4 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, mGFP tagged | 10 ug |
$1,392.00
|
|
| RC600005 | ERBB2 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), transcript variant 1, Signal peptide (1-22) plus EC domain (23-652) | 10 ug |
$910.00
|
|
| RG212583 | ERBB2 (tGFP-tagged) - Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$789.00
$1,292.00
|
|
| SC128161 | ERBB2 (untagged)-Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
| SC323549 | ERBB2 (untagged)-Kinase deficient mutant (K753M) of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
| SC323573 | ERBB2 (untagged)-Kinase deficient mutant (K753M) of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
Citations
Computational and Functional Analyses of HER2 Mutations Reveal Allosteric Activation Mechanisms and Altered Pharmacologic Effects
Ishiyama, O'Connor, Salomatov et al
Cancer Res (2022) 83 (9), 1531-1542 DOI: 10.1158/0008-5472.CAN-21-0940
Ishiyama, O'Connor, Salomatov et al
Cancer Res (2022) 83 (9), 1531-1542 DOI: 10.1158/0008-5472.CAN-21-0940
AI Summary: OriGene's Human HER2 cDNA (RC212583) was used as the template for generating HER2 mutants, enabling functional assays that identified and characterized oncogenic HER2 driver mutations in this study.
Snippet: … Retroviral production Human HER2 cDNA (RC212583;Origene) was subcloned into pMXs-IRES-Blasticidin (Cell Biolabs). HER2 mutants were generated by site-directed mutagenesis. Retroviral expression vector retrovirus was produced by transient transfecti;Human HER2 cDNA (RC212583;Origene, Rockville, MD) was subcloned into pMXs-IRES-Blasticidin (Cell Biolabs, San Diego, CA). HER2 mutants were generated by site-directed mutagenesis. …
Integrated approach to functional analysis of an ERBB2 variant of unknown significance detected by a cancer gene panel test
Harada, Sato, Araki et al
Cell Oncol (Dordr) (2022) 45 (1), 121-134 DOI: 10.1007/s13402-021-00656-3
Harada, Sato, Araki et al
Cell Oncol (Dordr) (2022) 45 (1), 121-134 DOI: 10.1007/s13402-021-00656-3
Cited by 2
AI Summary: OriGene products, specifically the Myc-DDK-tagged ORF clone of human HER2 (RC212583) and the empty vector (PS100001), were used as the basis for generating ERBB2 expression constructs to analyze the functional impact of ERBB2 variants in this study.
Snippet: … Plasmid constructs and transfection Myc-DDK-tagged ORF clones of human cDNAs encoding full-length HER2 (RC212583) and empty vector (PS100001) were obtained from Origene (Rockville, MD, USA). E401G, S310F, E321G and D845A mutations were introduced by using a QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and were ve;Myc-DDK-tagged ORF clones of human cDNAs encoding full-length HER2 (RC212583) and empty vector (PS100001) were obtained from Origene (Rockville, MD, USA)
BRET-based assay to monitor EGFR transactivation by the AT1R reveals Gq/11 protein-independent activation and AT1R-EGFR complexes
O'Brien, Johnstone, Devost et al
Biochem. Pharmacol. (2018)
O'Brien, Johnstone, Devost et al
Biochem. Pharmacol. (2018)
miR-125b acts as a tumor suppressor in chondrosarcoma cells by the sensitization to doxorubicin through direct targeting the ErbB2-regulated glucose metabolism
Tang, Zheng, Ding et al
Drug Des Devel Ther (2016) 10, 571-83 DOI: 10.2147/DDDT.S90530
Tang, Zheng, Ding et al
Drug Des Devel Ther (2016) 10, 571-83 DOI: 10.2147/DDDT.S90530
AI Summary: OriGene products contributed to this study by providing overexpression vectors for wild-type ErbB2 (RC212583) and LDHA (RC228293), which were used to investigate the roles of these genes in miR-125b-mediated sensitization of chondrosarcoma cells to doxorubicin.
Snippet: Transfection was performed using the Lipofectamine 2000 Transfection reagent (Invitrogen) according to the manufacturer's protocol. Overexpression vectors containing wild-type ErbB2 (RC212583) and LDHA (RC228293) were purchased from www.origene.com. ... Cite SaveSaving
Overexpression of ErbB2 renders breast cancer cells susceptible to 3-BrPA through the increased dissociation of hexokinase II from mitochondrial outer membrane
Gao, Chen, Jin et al
Oncol Lett (2016) 11 (2), 1567-1573 DOI: 10.3892/ol.2015.4043
Gao, Chen, Jin et al
Oncol Lett (2016) 11 (2), 1567-1573 DOI: 10.3892/ol.2015.4043
Cited by 16
AI Summary: OriGene products contributed to this study by providing the ErbB2 expression plasmid (Cat# RC217445) and ErbB2 shRNA plasmid (Cat# TG311793), which were used to manipulate ErbB2 levels in breast cancer cells and investigate its role in glycolysis and susceptibility to 3-BrPA.
Intra-tumor genetic heterogeneity and alternative driver genetic alterations in breast cancers with heterogeneous HER2 gene amplification
Ng, Martelotto, Gauthier et al
Genome Biol (2015) 16 (1), 107 DOI: 10.1186/s13059-015-0657-6
Ng, Martelotto, Gauthier et al
Genome Biol (2015) 16 (1), 107 DOI: 10.1186/s13059-015-0657-6
Cited by 137
AI Summary: In this study, OriGene products—including the BRF2 (RC202756) and DSN1 (RC220863) expression plasmids—were used to perform in vitro functional assays, helping to demonstrate that overexpression of BRF2 and DSN1 can drive tumorigenic features in HER2-negative breast cancer cells.
Showing 1-6 of 9 papers.
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