Her2 (ERBB2) (NM_004448) Human Untagged Clone
SKU
SC128161
ERBB2 (untagged)-Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1
$1,640.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | Her2 |
| Synonyms | CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_004448 |
| Insert Size | 3768 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | A TrueClone. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004448.2, NP_004439.2 |
| RefSeq Size | 4664 bp |
| RefSeq ORF | 3768 bp |
| Locus ID | 2064 |
| UniProt ID | P04626 |
| Cytogenetics | 17q12 |
| Domains | FU, Furin-like, pkinase, Recep_L_domain, S_TKc, TyrKc, YLP |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer |
| Summary | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. provided by RefSeq, Jul 2008 Transcript Variant: This variant (1) encodes the longest isoform (a). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004448" in other vectors (9)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212583 | ERBB2 (Myc-DDK-tagged)-Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,092.00
|
|
| RC212583L1 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,392.00
|
|
| RC212583L2 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, mGFP tagged | 10 ug |
$1,392.00
|
|
| RC212583L3 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,392.00
|
|
| RC212583L4 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, mGFP tagged | 10 ug |
$1,392.00
|
|
| RC600005 | ERBB2 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), transcript variant 1, Signal peptide (1-22) plus EC domain (23-652) | 10 ug |
$910.00
|
|
| RG212583 | ERBB2 (tGFP-tagged) - Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$789.00
$1,292.00
|
|
| SC323549 | ERBB2 (untagged)-Kinase deficient mutant (K753M) of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
| SC323573 | ERBB2 (untagged)-Kinase deficient mutant (K753M) of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
Citations
Erb‑B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53‑responsive microRNA‑3184‑5p in cervical cancer cells
Liu, Li, Zhang et al
Oncol Rep (2020) 45 (1), 95-106 DOI: 10.3892/or.2020.7862
Liu, Li, Zhang et al
Oncol Rep (2020) 45 (1), 95-106 DOI: 10.3892/or.2020.7862
Cited by 39
AI Summary: OriGene products, including ERBB2 expression plasmid (cat. no. SC128161), ERBB2-3'UTR wild-type plasmid (cat. no. SC208188), and the empty control vector (cat. no. PCMV6XL5), were used in this study to investigate the regulation and functional role of ERBB2 in cervical cancer cells.
Snippet: … SC116227) or ERBB2 (NM_004448;cat. … SC128161), and an empty negative control (NC) plasmid vector (cat. … PCMV6XL5) were obtained from OriGene Technologies, Inc no. SC208188) was obtained from OriGene Technologies, Inc;- plasmid vector (cat. … PCMV6XL5) were obtained from OriGene Technologies, Inc no. PS100062) containing the wild‑type (WT) 3'‑UTR of human ERBB2 (ERBB2‑3'‑UTRWT;cat. … SC208188) was obtained from OriGene Technologies, Inc
Hersintuzumab: A novel humanized anti-HER2 monoclonal antibody induces potent tumor growth inhibition
Amiri, Golsaz-Shirazi, Soltantoyeh et al
Invest New Drugs (2017)
Amiri, Golsaz-Shirazi, Soltantoyeh et al
Invest New Drugs (2017)
AI Summary: In this study, the HER2-pCMV-XL4 construct from OriGene was used as the template to amplify and subclone various HER2 extracellular domain subdomains, enabling the characterization of the novel anti-HER2 antibody, hersintuzumab.
Snippet: Briefly, recombi- nant full extracellular domain, single and paired subdomains of HER2-ECD, with 90 bp overlap, includ- ing DI, DII, DIII, DIV, DI + II and DIII + IV were amplified from HER2-pCMV-XL4 construct (OriGene Technologies, Rockville, MD, USA) and subcloned ...;... Briefly, recombi- nant full extracellular domain, single and paired subdomains of HER2-ECD, with 90 bp overlap, includ- ing DI, DII, DIII, DIV, DI + II and DIII + IV were amplified from HER2-pCMV-XL4 construct (OriGene Technologies, Rockville, MD, USA) and subcloned
Antibody Response to Human Extracellular HER2 Subdomain Proteins in Mice
Sadri-Ardalani, Ahmadi, Hemmati et al
Iran J Immunol (2017) 14 (2), 99-110 DOI: IJIv14i2A2
Sadri-Ardalani, Ahmadi, Hemmati et al
Iran J Immunol (2017) 14 (2), 99-110 DOI: IJIv14i2A2
AI Summary: OriGene’s HER2-pCMV-XL4 construct was used as the template to amplify and produce recombinant HER2 extracellular subdomains and full extracellular domain proteins, which were essential for evaluating antibody responses in the study.
Snippet: HER2-pCMV-XL4 (OriGene Technologies, Rockville, MD, USA) construct was employed as a template for the amplification of four HER2 extracellular subdomains and the full extracellular domain (fECD) using specific primers (Table 1). Domain I (DI) and DIII PCR products ... All 3 versions Cite SaveSaving
Antibody response to HER2 extracellular domain and subdomains in mouse following DNA immunization
Sadri-Ardalani, Shabani, Amiri et al
Tumour Biol. (2015)
Sadri-Ardalani, Shabani, Amiri et al
Tumour Biol. (2015)
Cited by 4
Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Gaborit, Larbouret, Vallaghe et al
J. Biol. Chem. (2011) 286 (13), 11337-45 DOI: 10.1074/jbc.M111.223503
Gaborit, Larbouret, Vallaghe et al
J. Biol. Chem. (2011) 286 (13), 11337-45 DOI: 10.1074/jbc.M111.223503
Cited by 13
Matrix metalloproteinase-1 expression can be upregulated through mitogen-activated protein kinase pathway under the influence of human epidermal growth factor receptor 2 synergized with estrogen receptor
Jung, Park, Jun et al
Mol Cancer Res (2010) 8 (7), 1037-47 DOI: 10.1158/1541-7786.MCR-09-0469
Jung, Park, Jun et al
Mol Cancer Res (2010) 8 (7), 1037-47 DOI: 10.1158/1541-7786.MCR-09-0469
AI Summary: OriGene products contributed to this paper by providing the ER-alpha (estrogen receptor alpha) and HER2 expression plasmids used to manipulate receptor levels in MCF-7 cells, enabling the study of their effects on MMP-1 expression.
Showing 1-6 of 6 papers.
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