Her2 (ERBB2) (NM_004448) Human Tagged ORF Clone
- TrueORF®
SKU
RG212583
ERBB2 (tGFP-tagged) - Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1
$789.00
$1,292.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Her2 |
| Synonyms | CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004448 |
| ORF Size | 3765 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004448.4 |
| RefSeq Size | 4624 bp |
| RefSeq ORF | 3768 bp |
| Locus ID | 2064 |
| UniProt ID | P04626 |
| Cytogenetics | 17q12 |
| Domains | FU, Furin-like, pkinase, Recep_L_domain, S_TKc, TyrKc, YLP |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer |
| Summary | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004448" in other vectors (9)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212583 | ERBB2 (Myc-DDK-tagged)-Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,092.00
|
|
| RC212583L1 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,392.00
|
|
| RC212583L2 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, mGFP tagged | 10 ug |
$1,392.00
|
|
| RC212583L3 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,392.00
|
|
| RC212583L4 | Lenti ORF clone of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, mGFP tagged | 10 ug |
$1,392.00
|
|
| RC600005 | ERBB2 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), transcript variant 1, Signal peptide (1-22) plus EC domain (23-652) | 10 ug |
$910.00
|
|
| SC128161 | ERBB2 (untagged)-Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
| SC323549 | ERBB2 (untagged)-Kinase deficient mutant (K753M) of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
| SC323573 | ERBB2 (untagged)-Kinase deficient mutant (K753M) of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 | 10 ug |
$1,640.00
|
|
Citations
Star-related lipid transfer protein 10 (STARD10): a novel key player in alcohol-induced breast cancer progression
Floris, Luo, Frank et al
J. Exp. Clin. Cancer Res. (2019) 38 (1), 4 DOI: 10.1186/s13046-018-1013-y
Floris, Luo, Frank et al
J. Exp. Clin. Cancer Res. (2019) 38 (1), 4 DOI: 10.1186/s13046-018-1013-y
AI Summary: OriGene products, specifically the Myc-DDK, pMIEG3-c-Jun, and pLX304-Fos-V5 plasmids along with their corresponding empty vector controls, were used for gene overexpression studies to investigate the roles of STARD10 and ERBB2 in alcohol-induced breast cancer progression.
Snippet: Myc-DDK, pMIEG3-c-Jun, pLX304-Fos-V5. All plasmids and the corresponding negative control empty vectors were purchased from Origene (Rockville, MD) and Addgene (Cambridge, MA). MCF-7 and SKBR-3 cells were cultured Cached
Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts
Kahl, Settele, Knick et al
Protein Expr. Purif. (2018)
Kahl, Settele, Knick et al
Protein Expr. Purif. (2018)
High cell-surface density of HER2 deforms cell membranes
Chung, Reichelt, Shao et al
Nat Commun (2016) 7, 12742 DOI: 10.1038/ncomms12742
Chung, Reichelt, Shao et al
Nat Commun (2016) 7, 12742 DOI: 10.1038/ncomms12742
Cited by 80
AI Summary: OriGene's HER2-GFP plasmid (RG212583) was used in this study to visualize and analyze the effects of HER2 overexpression on cell membrane deformation in breast cancer cells.
Snippet: lipophilic dye (DiI (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate, TF, D282;2 μM), c-terminus HER2 antibody (rabbit, Dako, A0485;1:2,000 dilution for WB), HER2-GFP (Origene, RG212583), αVillin (rabbit;Abcam, ab52102;1:50 dilution for IF),
Intra-tumor genetic heterogeneity and alternative driver genetic alterations in breast cancers with heterogeneous HER2 gene amplification
Ng, Martelotto, Gauthier et al
Genome Biol (2015) 16 (1), 107 DOI: 10.1186/s13059-015-0657-6
Ng, Martelotto, Gauthier et al
Genome Biol (2015) 16 (1), 107 DOI: 10.1186/s13059-015-0657-6
Cited by 137
AI Summary: In this study, OriGene products—including the BRF2 (RC202756) and DSN1 (RC220863) expression plasmids—were used to perform in vitro functional assays, helping to demonstrate that overexpression of BRF2 and DSN1 can drive tumorigenic features in HER2-negative breast cancer cells.
Showing 1-4 of 4 papers.
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