CBS (NM_000071) Human Tagged ORF Clone
$513.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CBS |
| Synonyms | CBSL; HIP4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000071 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000071.3 |
| RefSeq Size | 2609 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 875 |
| UniProt ID | P35520 |
| Cytogenetics | 21q22.3 |
| Domains | CBS, PALP |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Glycine, Metabolic pathways, Selenoamino acid metabolism, serine and threonine metabolism |
| MW | 60.6 kDa |
| Summary | The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Feb 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000071" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201755L1 | Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, Myc-DDK-tagged | 10 ug |
$813.00
|
|
| RC201755L2 | Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, mGFP tagged | 10 ug |
$813.00
|
|
| RC201755L3 | Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, Myc-DDK-tagged | 10 ug |
$813.00
|
|
| RC201755L4 | Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, mGFP tagged | 10 ug |
$813.00
|
|
| RG201755 | CBS (tGFP-tagged) - Human cystathionine-beta-synthase (CBS), transcript variant 1 | 10 ug |
$489.00
$713.00
|
|
| SC128074 | CBS (untagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1 | 10 ug |
$514.00
|
|
| SC324364 | CBS (untagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1 | 10 ug |
$514.00
|
|
Citations
A highly selective and sensitive near-infrared fluorescent probe for imaging of hydrogen sulphide in living cells and mice
Zhang, Zheng, Zou et al
Sci Rep (2016) 6, 18868 DOI: 10.1038/srep18868
Zhang, Zheng, Zou et al
Sci Rep (2016) 6, 18868 DOI: 10.1038/srep18868
Cited by 83
AI Summary: OriGene contributed to this study by providing the human CBS cDNA clone (lot no.: RC201755), which was used to generate CBS-overexpressing cells for investigating endogenous hydrogen sulphide production.
Snippet: Protein bands were scanned and quantified by densitometric analysis using ImageJ version 1.34 s software. Construction of CBS overexpressing cells. The cDNA clones for human CBS were purchased from Origene (lot no.: RC201755). ... Cite SaveSaving
Two-Photon Fluorescent Probe for Detection of Exogenous and Endogenous Hydrogen Persulfide and Polysulfide in Living Organisms
Zeng, Chen, Xia et al
Anal. Chem. (2015)
Zeng, Chen, Xia et al
Anal. Chem. (2015)
Cited by 117
Showing 1-2 of 2 papers.
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