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CBS (NM_000071) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC201755L4
Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $813.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CBS
Synonyms CBSL; HIP4
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC201755).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000071
ORF Size 1653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000071.1
RefSeq Size 2609 bp
RefSeq ORF 1656 bp
Locus ID 875
UniProt ID P35520
Cytogenetics 21q22.3
Domains CBS, PALP
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Glycine, Metabolic pathways, Selenoamino acid metabolism, serine and threonine metabolism
MW 60.6 kDa
Summary The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Feb 2016
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"NM_000071" in other vectors (7)
SKU Description Size Price
RC201755 CBS (Myc-DDK-tagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1 10 ug
$513.00
RC201755L1 Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, Myc-DDK-tagged 10 ug
$813.00
RC201755L2 Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, mGFP tagged 10 ug
$813.00
RC201755L3 Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, Myc-DDK-tagged 10 ug
$813.00
RG201755 CBS (tGFP-tagged) - Human cystathionine-beta-synthase (CBS), transcript variant 1 10 ug
$489.00 $713.00
SC128074 CBS (untagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1 10 ug
$514.00
SC324364 CBS (untagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1 10 ug
$514.00

Plasmid Map for RC201755L4

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Cloning scheme for RC201755L4
Circular map for RC201755L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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