CBS (NM_000071) Human Untagged Clone

SKU
SC128074
CBS (untagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1
$514.00
2 Weeks*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CBS
Synonyms CBSL; HIP4
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000071.1 CAGCCGCTCGCCCCTCTTTTCCATGTATCCGTCCAGGATCCCATGACAGATTCTGTTGTC
ACGTCTCCTTACAGAGTTTGAGCGGTGCTGAACTGTCAGCACCATCTGTCCGGTCCCAGC
ATGCCTTCTGAGACCCCCCAGGCAGAAGTGGGGCCCACAGGCTGCCCCCACCGCTCAGGG
CCACACTCGGCGAAGGGGAGCCTGGAGAAGGGGTCCCCAGAGGATAAGGAAGCCAAGGAG
CCCCTGTGGATCCGGCCCGATGCTCCGAGCAGGTGCACCTGGCAGCTGGGCCGGCCTGCC
TCCGAGTCCCCACATCACCACACTCCCCCGGCAAAATCTCCAAAAATCTTGCCAGATATT
CTGAAGAAAATCGGGGACACCCCTATGGTCAGAATCAACAAGATTGGGAAGAAGTTCGGC
CTGAAGTGTGAGCTCTTGGCCAAGTGTGAGTTCTTCAACGCGGGCGGGAGCGTGAAGGAC
CGCATCAGCCTGCGGATGATTGAGGATGCTGAGCGCGACGGGACGCTGAAGCCCGGGGAC
ACGATTATCGAGCCGACATCCGGGAACACCGGGATCGGGCTGGCCCTGGCTGCGGCAGTG
AGGGGCTATCGCTGCATCATCGTGATGCCAGAGAAGATGAGCTCCGAGAAGGTGGACGTG
CTGCGGGCACTGGGGGCTGAGATTGTGAGGACGCCCACCAATGCCAGGTTCGACTCCCCG
GAGTCACACGTGGGGGTGGCCTGGCGGCTGAAGAACGAAATCCCCAATTCTCACATCCTA
GACCAGTACCGCAACGCCAGCAACCCCCTGGCTCACTACGACACCACCGCTGATGAGATC
CTGCAGCAGTGTGATGGGAAGCTGGACATGCTGGTGGCTTCAGTGGGCACGGGCGGCACC
ATCACGGGCATTGCCAGGAAGCTGAAGGAGAAGTGTCCTGGATGCAGGATCATTGGGGTG
GATCCCGAAGGGTCCATCCTCGCAGAGCCGGAGGAGCTGAACCAGACGGAGCAGACAACC
TACGAGGTGGAAGGGATCGGCTACGACTTCATCCCCACGGTGCTGGACAGGACGGTGGTG
GACAAGTGGTTCAAGAGCAACGATGAGGAGGCGTTCACCTTTGCCCGCATGCTGATCGCG
CAAGAGGGGCTGCTGTGCGGTGGCAGTGCTGGCAGCACGGTGGCGGTGGCCGTGAAGGCC
GCGCAGGAGCTGCAGGAGGGCCAGCGCTGCGTGGTCATTCTGCCCGACTCAGTGCGGAAC
TACATGACCAAGTTCCTGAGCGACAGGTGGATGCTGCAGAAGGGCTTTCTGAAGGAGGAG
GACCTCACGGAGAAGAAGCCCTGGTGGTGGCACCTCCGTGTTCAGGAGCTGGGCCTGTCA
GCCCCGCTGACCGTGCTCCCGACCATCACCTGTGGGCACACCATCGAGATCCTCCGGGAG
AAGGGCTTCGACCAGGCGCCCGTGGTGGATGAGGCGGGGGTAATCCTGGGAATGGTGACG
CTTGGGAACATGCTCTCGTCCCTGCTTGCCGGGAAGGTGCAGCCGTCAGACCAAGTTGGC
AAAGTCATCTACAAGCAGTTCAAACAGATCCGCCTCACGGACACGCTGGGCAGGCTCTCG
CACATCCTGGAGATGGACCACTTCGCCCTGGTGGTGCACGAGCAGATCCAGTACCACAGC
ACCGGGAAGTCCAGTCAGCGGCAGATGGTGTTCGGGGTGGTCACCGCCATTGACTTGCTG
AACTTCGTGGCCGCCCAGGAGCGGGACCAGAAGTGAAGTCCGGAGAGCTGGGCGGTGCGG
AGCGGGCCCGCCACCCTTGCCCACTTCTCCTTCGCTTTCCTGAGCCCTAAACACACGCGT
GATTGGTAACTGCCTGGCACCGTTATCCCTGCAGACGGCACAGAGCATCCGTCTCCCCTC
GTTAACACATGGCTTCCTAAATGGCCCTGTTTACGGCCTATGAGATGAAATATGTGATTT
TCTCTAATGTAACTTCCTCTTAGGATGTTTCACCAAGGAAATATTGAGAGAGAAGTCGGC
CAGGTAGGATGAACACAGGCAATGACTGCACAGAGTGGATTAAAGGCAAAAGAGAGAAGA
GTCCAGGAAGGGGCGGGGAGAAGCCTGGGTGGCTCAGCATCCTCCACGGGCTGCGCCGTC
TGCTCGGGGCTGAGCTGGCGGGAGCAGTTTGCGTGTTTGGGTTTTTTAATTGAGATGAAA
TTCAAATAACCTAAAAATCAATCACTTGAAAGTGAACAATCAGCGGCATTTAGTACATCC
AGAAAGTTGTGTAGGCACCACCTCTGTCACGTTCTGGAACATTCTGTCATCACCCCGTGA
AGCAATCATTTCCCCTCCCGTCTTCCTCCTCCCCTGGCAACTGCTGATCGACTTTGTGTC
TCTGTTGTCTAAAATAGGTTTTCCCTGTTCTGGACATTTCATATAAATGGAATCACACAA
AAAAAAAAAAAAAAAAAAA
Restriction Sites NotI-NotI
ACCN NM_000071
Insert Size 2360 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000071.1, NP_000062.1
RefSeq Size 2544 bp
RefSeq ORF 1656 bp
Locus ID 875
UniProt ID P35520
Cytogenetics 21q22.3
Domains CBS, PALP
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Glycine, Metabolic pathways, Selenoamino acid metabolism, serine and threonine metabolism
Summary The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2016]
Write Your Own Review
You're reviewing:CBS (NM_000071) Human Untagged Clone
Your Rating
SKU Description Size Price
RC201755 CBS (Myc-DDK-tagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1 10 ug
$513.00
RC201755L1 Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, Myc-DDK-tagged 10 ug
$813.00
RC201755L2 Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, mGFP tagged 10 ug
$813.00
RC201755L3 Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, Myc-DDK-tagged 10 ug
$813.00
RC201755L4 Lenti ORF clone of Human cystathionine-beta-synthase (CBS), transcript variant 1, mGFP tagged 10 ug
$813.00
RG201755 CBS (tGFP-tagged) - Human cystathionine-beta-synthase (CBS), transcript variant 1 10 ug
$489.00 MSRP $713.00 MSRP $713.00
SC324364 CBS (untagged)-Human cystathionine-beta-synthase (CBS), transcript variant 1 10 ug
$514.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.