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pCMV6-AC Mammalian Expression Vector

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SKU
PS100020
pCMV6-AC, mammalian vector with non-tagged expression, 10ug
  $470.00
In Stock*

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Specifications
Specifications
Product Data
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • This vector is essentially the same as the pCMV6-Entry vector, except for the absence of the C-terminal Myc-DDK tag
  • ORF cloned into this vector at SgfI and RsrII sites will express the ORF-encoded protein plus two additional amino acids at the C-terminus, T and R
  • The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein
  • V2_F Primer can be used to sequence the target sequence after cloning
  • XL39_R Primer can be used to sequence the target sequence after cloning
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

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Inactivation of the Phosphatase Activity of Soluble Epoxide Hydrolase Modulates SIRT3 and Attenuates Experimental Pulmonary Hypertension
Leuillier, Chelgham, Messaoudi et al
Compr Physiol (2026) 16 (1), e70108 DOI: 10.1002/cph4.70108
Cited by 1
AI Summary: OriGene’s pCMV6-Entry mammalian expression vector (PS100020) was used to modulate gene expression in cultured human pulmonary artery smooth muscle cells, enabling the investigation of soluble epoxide hydrolase’s role in pulmonary hypertension.
Snippet: , Rockville, MD, USA) or 1 ng of the pCMV6-Entry mammalian expression vector (p-Ctr, PS100020;OriGene). All experiments were performed 48 h after transfection
FigureFigureFigure
Modulating ACVRL1 Expression in HMEC1 Cells as a Simplified In Vitro Model for Hereditary Hemorrhagic Telangiectasia (HHT) Type 2 Studies
Rusche, Bette, Stuck et al
In Vivo (2026) 40 (1), 64-75 DOI: 10.21873/invivo.14173
AI Summary: OriGene products contributed to this study by providing the ACVRL1-wildtype encoding plasmid (pCMV6-AC ACVRL1, #SC321860) and the corresponding empty vector (pCMV6-AC, #PS100020), which were used for transfection experiments to assess the effects of ACVRL1 expression in HMEC-1 cells.
Snippet: ested for transfection and incubated in 390 µl hypoosmolar buffer (90 mOsmol/kg, #732-6007, Eppendorf) together with 8 µg of an ACVRL1-wildtype encoding plasmid (pCMV6-AC ACVRL1, #SC321860, Origene, Rockville, FL, USA). The respective empty vector (pCMV6-AC, #PS100020
FigureFigureFigure
Depletion of RBMS1 alleviates oxaliplatin chemoresistance in gastric cancer cells by suppressing the efflux function of ABCB1
Yang, Zhang, Chen et al
European Journal of Medical Research (2026)
AI Summary: OriGene products, including the RBMS1 human expression vector (SC319246), control vector (PS100020), RBMS1 Human siRNA Oligo Duplex (SR304012), EIF3D Human siRNA Oligo Duplex (SR305698), and control siRNA (SR30002), were used to modulate gene expression and investigate the role of RBMS1 and EIF3D in oxaliplatin chemoresistance in gastric cancer cells.
Snippet: The RBMS1 human expression vector (Cat. … SC319246) and the control vector (Cat. … PS100020) were purchased from OriGene (Rockville, MD, USA). RBMS1 Human siRNA Oligo Duplex (Cat. … SR304012), EIF3D Human siRNA Oligo Duplex (Cat. … SR305698) and the control siRNA (Cat. … SR30002) were purchased from OriGene (Rockville, MD, USA). …
Unraveling Cathepsin S regulation in interleukin-7-mediated anti-tumor immunity reveals its targeting potential against oral cancer
Chang, Chen, Chen et al
J Biomed Sci (2025) 32 (1), 69 DOI: 10.1186/s12929-025-01154-6
Cited by 2
AI Summary: OriGene products pCMV6-AC-CTSS (cat# sc319097) and pCMV6-AC (cat# ps100020) were used for CTSS overexpression and as a control plasmid, respectively, to investigate the role of CTSS in regulating IL-7-mediated anti-tumor immunity in oral cancer.
Snippet: at room temperature, followed by measuring the absorbance of the solution at 450 nm wavelength by TECAN reader to quantify the IL-7 amount. Transfection of DNA plasmids pCMV6-AC-CTSS (cat#sc319097;Origene, Rockville, MD, USA), pCMV6-AC (cat#ps100020;Origene), mouse CTSS shRNA plasmid (m) (cat#sc-29941-SH;Santa Cruz), and control shRNA plasmid-A (a control plasmid DNA that expresses non-target
FigureFigureFigure
Mitochondrial transport of glutathione in diabetic retinopathy
Kowluru, Malaviya
Free Radic Biol Med (2025) 237, 357-368 DOI: 10.1016/j.freeradbiomed.2025.06.004
Cited by 7
AI Summary: In this study, OriGene’s TurboFectin 8.0 transfection reagent (Cat no. TF81001) was used for plasmid delivery, and the pCMV6-AC empty vector (Cat no. PS100020) served as a negative control, enabling researchers to efficiently manipulate gene expression to investigate mitochondrial glutathione transport in diabetic retinopathy.
Snippet: ssion, HRECs were transfected with plasmid constructs overexpressing DIC- or OGC- (GenScript, Piscataway, NJ, USA), cloned in pcDNA 3.1 using TurboFectin 8.0 transfection reagent (Cat no. TF81001, OriGene Technologies, Rockville, MD, USA). … PS100020, OriGene Technologies) were used as negative controls. The transfection efficiency was accessed by quantitative real-time PCR [18,26,27]. …
Transcription factor ETV4 plays a critical role in the development of non-alcoholic fatty liver disease
Gadiraju, Magisetty, Kondreddy
Int J Biol Macromol (2024) 282 (Pt 5), 137235 DOI: 10.1016/j.ijbiomac.2024.137235
Cited by 8
AI Summary: OriGene contributed to this study by providing the pCMV-AC-human ANGPTL4 plasmid clone (#SC320482) and the pCMV6-AC empty mammalian vector (#PS100020), which were used to investigate the molecular mechanisms underlying the role of ETV4 in non-alcoholic fatty liver disease.
Snippet: The pCMV-AC- human ANGPTL4 plasmid clone (#SC320482) and pCMV6-AC empty mammalian vector (#PS100020) were purchased from Origene, MD, USA. The βarrestin 1 antibody was procured from Santa Cruz Biotechnology, USA (#sc-9182). …
Showing 1-6 of 26 papers.
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