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pCas-Guide-Nickase (D10A)

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SKU
GE100019
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 D10A expression) (10 µg)
  $670.00
In Stock*

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TP790151 Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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Specifications
Specifications
Product Data
Function Cas9 Nickase
Features

All-in-one vector

  • Expresses human codon-optimized Cas9 nickase D10A mutant, C-terminal Myc-DDK tagged
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
  • CF3 primer can be used to sequence the target sequence after cloning
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing
Noman, Aqeel, He
Front Plant Sci (2016) 7, 1740 DOI: 10.3389/fpls.2016.01740
Cited by 93
AI Summary: In the paper "CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing," OriGene products such as the Cas9 expression plasmid and guide RNA cloning kits were utilized to facilitate efficient CRISPR-Cas9-mediated genome editing in plants, enabling targeted gene modifications discussed in the review.
FigureFigureFigure
Showing 1-1 of 1 papers.
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