FYN (NM_002037) Human Recombinant Protein
SKU
TP324691
Recombinant protein of human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1, 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 60.6 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | FYN activity verified in a biochemical assay: FYN (FYN oncogene related to SRC, FGR, YES) (TP324691) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FYN is a non-receptor tyrosine kinase and is a member of the Src kinase family consisting of Src, Fyn, Yes, Lck, Lyn, Hck, Fgr and Blk. Varying concentrations of FYN were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “&DeltaR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors. |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_002028 |
| Locus ID | 2534 |
| UniProt ID | P06241 |
| Cytogenetics | 6q21 |
| RefSeq Size | 2650 |
| RefSeq ORF | 1611 |
| Synonyms | p59-FYN; SLK; SYN |
| Summary | This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. provided by RefSeq, Jul 2008 |
| Protein Categories | Enzyme: Kinases, Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "FYN" proteins (11)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH307620 | FYN MS Standard C13 and N15-labeled recombinant protein (NP_694593) | 10 ug |
$3,360.00
|
|
| PH319374 | FYN MS Standard C13 and N15-labeled recombinant protein (NP_694592) | 10 ug |
$3,360.00
|
|
| PH324691 | FYN MS Standard C13 and N15-labeled recombinant protein (NP_002028) | 10 ug |
$3,360.00
|
|
| LC400745 | Lyophilized FYN HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC403505 | Lyophilized FYN HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC407122 | Lyophilized FYN HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400745 | Transient overexpression lysate of FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| LY403505 | Transient overexpression lysate of FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
| LY407122 | Transient overexpression lysate of FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| TP307620 | Recombinant protein of human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 3, 20 µg | 20 ug |
$737.00
|
|
| TP319374 | Recombinant protein of human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 2, 20 µg | 20 ug |
$737.00
|
|
Citations
Two novel ligand-independent variants of the VEGFR-1 receptor are expressed in human testis and spermatozoa, one of them with the ability to activate SRC proto-oncogene tyrosine kinases
Alvarez-Palomo, Barrot-Feixat, Sarret et al
Oncotarget (2019) 10 (56), 5871-5887 DOI: 10.18632/oncotarget.27232
Alvarez-Palomo, Barrot-Feixat, Sarret et al
Oncotarget (2019) 10 (56), 5871-5887 DOI: 10.18632/oncotarget.27232
Snippet: :"JF509745","term_id":"379023470","term_text":"JF509745"}}JF509745 [/nuccore/JF509745]. RECOMBINANT CLONES, CLONING, ELECTRO-TRANSFORMATION, AND DNA PREPARATION Human Src recombinant clone was from OriGene Technologies and contained v-Src sarcoma transcript variant 1 ({"type":"entrez-nucleotide","attrs":{"text":"NM_005417","term_id":"520262038","term_text":"NM_005417"}}NM_005417 [/nuccore/NM_005
Showing 1-1 of 1 papers.
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