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FYN (NM_002037) Human Over-expression Lysate

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SKU
LY400745
Transient overexpression lysate of FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1 (as WB positive control)
  $665.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224691
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002037, NP_002028
Synonyms p59-FYN; SLK; SYN
Predicted MW 60.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002028
Locus ID 2534
Cytogenetics 6q21
Protein Families WB
Protein Pathways Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis
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Other "FYN" proteins (11)
SKU Description Size Price
PH307620 FYN MS Standard C13 and N15-labeled recombinant protein (NP_694593) 10 ug
$3,360.00
PH319374 FYN MS Standard C13 and N15-labeled recombinant protein (NP_694592) 10 ug
$3,360.00
PH324691 FYN MS Standard C13 and N15-labeled recombinant protein (NP_002028) 10 ug
$3,360.00
LC400745 Lyophilized FYN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC403505 Lyophilized FYN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407122 Lyophilized FYN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403505 Transient overexpression lysate of FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY407122 Transient overexpression lysate of FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP307620 Recombinant protein of human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 3, 20 µg 20 ug
$737.00
TP319374 Recombinant protein of human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 2, 20 µg 20 ug
$737.00
TP324691 Recombinant protein of human FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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