IDE (NM_004969) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 117.8 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004960 |
| Locus ID | 3416 |
| UniProt ID | P14735 |
| Cytogenetics | 10q23.33 |
| RefSeq Size | 3279 |
| RefSeq ORF | 3057 |
| Synonyms | INSULYSIN |
| Summary | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.provided by RefSeq, Sep 2009 |
| Protein Categories | Enzyme: Peptidases, Intracellular Proteins, Secreated Proteins |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Alzheimer's disease |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "IDE" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH320700 | IDE MS Standard C13 and N15-labeled recombinant protein (NP_004960) | 10 ug |
$3,360.00
|
|
| LC401545 | Lyophilized IDE HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401545 | Transient overexpression lysate of insulin-degrading enzyme (IDE), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| TP721043 | Purified recombinant protein of Human insulin-degrading enzyme (IDE), transcript variant 1 | 10 ug |
$340.00
|
|
| TP724587 | Recombinant human Insulysin/IDE protein | 10 ug |
$325.00
|
|
Citations
The core sequence of PIF competes for insulin/amyloid β in insulin degrading enzyme: potential treatment for Alzheimer's disease
Hayrabedyan, Todorova, Spinelli et al
Oncotarget (2018) 9 (74), 33884-33895 DOI: 10.18632/oncotarget.26057
Hayrabedyan, Todorova, Spinelli et al
Oncotarget (2018) 9 (74), 33884-33895 DOI: 10.18632/oncotarget.26057
Snippet: -3 at a concentration of 10 μM in buffer containing 10 mM HEPES-Na (pH 7.5) and 100 mM NaCl in a 50-μL volume was mixed with recombinant IDE protein (OriGene ID Save;;As ligands peptide solutions of PIF (wt sequence), PIF mut-1 , and PIF mut-3 at a concentration of 10 μM in buffer containing 10 mM HEPES-Na (pH 7.5) and 100 mM NaCl in a 50-μL volume was mixed with recombinant IDE protein (OriGene ID TP320700) at concentration
Analysis of the proteolysis of bioactive peptides using a peptidomics approach
Kim, Lone, Saghatelian
Nat Protoc (2013) 8 (9), 1730-42 DOI: 10.1038/nprot.2013.104
Kim, Lone, Saghatelian
Nat Protoc (2013) 8 (9), 1730-42 DOI: 10.1038/nprot.2013.104
Cited by 28
AI Summary: OriGene products, specifically the insulin-degrading enzyme (IDE) cDNA (Cat# SC119387) and IDE shRNA plasmids (Cat# TL506959), were used in this study to overexpress or knock down IDE in cells, enabling the authors to investigate and validate the role of IDE in the proteolysis of bioactive peptides.
Showing 1-2 of 2 papers.
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