IDE (42-1019, His-Tag) Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | Baculovirus |
| Expression cDNA Clone or AA Sequence | |
| Tag | His-Tag |
| Predicted MW | 114kDa (984aa) |
| Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
| Purity | > 90% by SDS-PAGE |
| Buffer | Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 0.05% Brij35, 10% glycerol |
| Bioactivity | Specific activity is > 3,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK(Dnp)-OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25°C. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Recommended Dilution | SDS-PAGE, Enzyme Activity |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_004960.2 |
| UniProt ID | P14735 |
| Synonyms | Insulin-degrading enzyme, insulin-degrading enzyme isoform 1, Abeta-degrading protease, Insulin protease, Insulysin, IDE |
| Summary | Insulysin/IDE, also known insulin-degrading enzyme, is a zinc metallopeptidase of the inverzincin family. This enzyme catalyses the degradation reaction of insulin, glucagon and other polypeptides. It is present in mammals and in many arthropods such as the fly Drosophila melanogaster. It is also expressed in many tissues, with the highest levels in liver, kidney, brain, and testis. It has been shown to degrade the amyloid beta peptide, which polymerizes into the plaques associated with Alzheimers disease. Therefore, the deficiency of IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus and Alzheimers disease. Recombinant human Insulysin/IDE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Protein Categories | Enzyme: Peptidases, Intracellular Proteins, Secreated Proteins |
| Protein Families | Enzyme Activity, SDS-PAGE |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH320700 | IDE MS Standard C13 and N15-labeled recombinant protein (NP_004960) | 10 ug |
$3,360.00
|
|
| LC401545 | Lyophilized IDE HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401545 | Transient overexpression lysate of insulin-degrading enzyme (IDE), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| TP320700 | Recombinant protein of human insulin-degrading enzyme (IDE), 20 µg | 20 ug |
$737.00
|
|
| TP721043 | Purified recombinant protein of Human insulin-degrading enzyme (IDE), transcript variant 1 | 10 ug |
$340.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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