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EZH2 (NM_152998) Human Over-expression Lysate

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SKU
LY430254
Transient overexpression lysate of enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 2 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216079
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_152998, NP_694543
Synonyms ENX-1; ENX1; EZH2b; KMT6; KMT6A; WVS; WVS2
Predicted MW 80.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_694543
Locus ID 2146
Cytogenetics 7q36.1
Protein Families WB
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Other "EZH2" proteins (7)
SKU Description Size Price
PH302054 EZH2 MS Standard C13 and N15-labeled recombinant protein (NP_004447) 10 ug
$3,360.00
LC401416 Lyophilized EZH2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC407239 Lyophilized EZH2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430254 Lyophilized EZH2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401416 Transient overexpression lysate of enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY407239 Transient overexpression lysate of enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP302054 Recombinant protein of human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

miR-21 modulates the effect of EZH2 on the biological behavior of human lung cancer stem cells in vitro
Xia, Zhang, Zhang et al
Oncotarget (2017) 8 (49), 85442-85451 DOI: 10.18632/oncotarget.20006
Cited by 23
AI Summary: OriGene's EZH2-mutant (NM_152998) expression constructs were used in this study to manipulate EZH2 levels in lung cancer stem cells, enabling the authors to assess the functional relationship between EZH2 and miR-21 in regulating cancer cell behavior.
Snippet: Furthermore, EZH2-mutant (NM_ 152998, Origene, Beijing, China) expression constructs were generated by cloning the corresponding cDNAs into the vector. At 6 h post-transfection, the medium was replaced with standard culture medium
FigureFigureFigure
MicroRNA-138 suppresses epithelial-mesenchymal transition in squamous cell carcinoma cell lines
Liu, Wang, Chen et al
Biochem J (2011) 440 (1), 23-31 DOI: 10.1042/BJ20111006
AI Summary: In this study, OriGene products—including the Human VIM (vimentin) cDNA clone and EZH2 shRNA lentiviral particles—were used to investigate and validate the roles of miR-138 target genes in regulating epithelial-mesenchymal transition in squamous cell carcinoma cell lines.
FigureFigure
Showing 1-2 of 2 papers.
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