EZH2 (NM_152998) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC216079 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_152998, NP_694543 |
| Synonyms | ENX-1; ENX1; EZH2b; KMT6; KMT6A; WVS; WVS2 |
| Predicted MW | 80.9 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_694543 |
| Locus ID | 2146 |
| Cytogenetics | 7q36.1 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "EZH2" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH302054 | EZH2 MS Standard C13 and N15-labeled recombinant protein (NP_004447) | 10 ug |
$3,360.00
|
|
| LC401416 | Lyophilized EZH2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC407239 | Lyophilized EZH2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401416 | Transient overexpression lysate of enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY407239 | Transient overexpression lysate of enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
| LY430254 | Transient overexpression lysate of enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 2 (as WB positive control) | 100 ug |
$665.00
|
|
| TP302054 | Recombinant protein of human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, 20 µg | 20 ug |
$867.00
|
|
Citations
miR-21 modulates the effect of EZH2 on the biological behavior of human lung cancer stem cells in vitro
Xia, Zhang, Zhang et al
Oncotarget (2017) 8 (49), 85442-85451 DOI: 10.18632/oncotarget.20006
Xia, Zhang, Zhang et al
Oncotarget (2017) 8 (49), 85442-85451 DOI: 10.18632/oncotarget.20006
Cited by 23
AI Summary: OriGene's EZH2-mutant (NM_152998) expression constructs were used in this study to manipulate EZH2 levels in lung cancer stem cells, enabling the authors to assess the functional relationship between EZH2 and miR-21 in regulating cancer cell behavior.
Snippet: Furthermore, EZH2-mutant (NM_ 152998, Origene, Beijing, China) expression constructs were generated by cloning the corresponding cDNAs into the vector. At 6 h post-transfection, the medium was replaced with standard culture medium
MicroRNA-138 suppresses epithelial-mesenchymal transition in squamous cell carcinoma cell lines
Liu, Wang, Chen et al
Biochem J (2011) 440 (1), 23-31 DOI: 10.1042/BJ20111006
Liu, Wang, Chen et al
Biochem J (2011) 440 (1), 23-31 DOI: 10.1042/BJ20111006
AI Summary: In this study, OriGene products—including the Human VIM (vimentin) cDNA clone and EZH2 shRNA lentiviral particles—were used to investigate and validate the roles of miR-138 target genes in regulating epithelial-mesenchymal transition in squamous cell carcinoma cell lines.
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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