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FAK (PTK2) (NM_005607) Human Over-expression Lysate

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SKU
LY429259
Transient overexpression lysate of PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 2 (as WB positive control)
  $665.00
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Other products for "FAK or NM_005607"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221398
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005607, NP_005598
Synonyms FADK; FADK 1; FAK; FAK1; FRNK; p125FAK; pp125FAK; PPP1R71
Predicted MW 121.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005598
Locus ID 5747
Cytogenetics 8q24.3
Protein Families WB
Protein Pathways Axon guidance, Chemokine signaling pathway, ErbB signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer, VEGF signaling pathway
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Other "FAK" proteins (9)
SKU Description Size Price
PH319839 PTK2 MS Standard C13 and N15-labeled recombinant protein (NP_722560) 10 ug
$3,360.00
PH321398 PTK2 MS Standard C13 and N15-labeled recombinant protein (NP_005598) 10 ug
$3,360.00
LC403521 Lyophilized PTK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417186 Lyophilized PTK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429259 Lyophilized PTK2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403521 Transient overexpression lysate of PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY417186 Transient overexpression lysate of PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP319839 Recombinant protein of human PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 1, 20 µg 20 ug
$867.00
TP321398 Recombinant protein of human PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 2, 20 µg 20 ug
$867.00

file-alt Citations

Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
Soler, Estevez, Alvarez et al
Sensors (Basel) (2014) 14 (2), 2239-58 DOI: 10.3390/s140202239
Cited by 81
AI Summary: In this study, OriGene's Focal Adhesion Kinase (FAK) protein was used as a biomarker target to evaluate and optimize site-specific antibody immobilization strategies for direct detection in biosensor applications.
Snippet: Human Chorionic Gonadotropin (hCG) was obtained from Abcam (Cambridge, UK), Focal Adhesion Kinase (FAK) was obtained from OriGene (Rockville, MD, USA) and C-Reactive Protein (CRP) from AntibodyBcn (Barcelona, Spain). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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