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WDR79 (WRAP53) (NM_001143991) Human Over-expression Lysate

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SKU
LY428451
Transient overexpression lysate of WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3 (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC227773
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001143991, NP_001137463
Synonyms DKCB3; TCAB1; WDR79
Predicted MW 59.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001137463
Locus ID 55135
Cytogenetics 17p13.1
Protein Families WB
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Other "WDR79" proteins (15)
SKU Description Size Price
PH300142 WRAP53 MS Standard C13 and N15-labeled recombinant protein (NP_060551) 10 ug
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PH327755 WRAP53 MS Standard C13 and N15-labeled recombinant protein (NP_001137462) 10 ug
$3,360.00
PH327773 WRAP53 MS Standard C13 and N15-labeled recombinant protein (NP_001137463) 10 ug
$3,360.00
PH327786 WRAP53 MS Standard C13 and N15-labeled recombinant protein (NP_001137464) 10 ug
$3,360.00
LC413323 Lyophilized WRAP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428450 Lyophilized WRAP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428451 Lyophilized WRAP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428452 Lyophilized WRAP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY413323 Transient overexpression lysate of WD repeat containing, antisense to TP53 (WRAP53), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY428450 Transient overexpression lysate of WD repeat containing, antisense to TP53 (WRAP53), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY428452 Transient overexpression lysate of WD repeat containing, antisense to TP53 (WRAP53), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP300142 Recombinant protein of human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 1, 20 µg 20 ug
$737.00
TP327755 Purified recombinant protein of Homo sapiens WD repeat containing, antisense to TP53 (WRAP53), transcript variant 2, 20 µg 20 ug
$737.00
TP327773 Purified recombinant protein of Homo sapiens WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3, 20 µg 20 ug
$737.00
TP327786 Purified recombinant protein of Homo sapiens WD repeat containing, antisense to TP53 (WRAP53), transcript variant 4, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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