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WDR79 (WRAP53) (NM_001143991) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC227773
WRAP53 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $510.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDR79
Synonyms DKCB3; TCAB1; WDR79
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC227773 representing NM_001143991
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACTTTGGAGACTCAACCGTTAGCTCCGGACTGCTGTCCTTCAGACCAGGACCCAGCTCCAGCCC
ATCCTTCTCCCCACGCTTCCCCGATGAATAAAAATGCGGACTCTGAACTGATGCCACCGCCTCCCGAAAG
GGGGGATCCGCCCCGGTTGTCCCCAGATCCTGTGGCTGGCTCAGCTGTGTCCCAGGAGCTACGGGAGGGG
GACCCAGTTTCTCTCTCCACTCCCCTGGAAACAGAGTTTGGTTCCCCTAGTGAGTTGAGTCCTCGAATCG
AGGAGCAAGAACTTTCTGAAAATACAAGCCTTCCTGCAGAAGAAGCAAACGGGAGCCTTTCTGAAGAAGA
AGCGAACGGGCCAGAGTTGGGGTCTGGAAAAGCCATGGAAGATACCTCTGGGGAACCCGCTGCAGAGGAC
GAGGGAGACACCGCTTGGAACTACAGCTTCTCCCAGCTGCCTCGATTTCTCAGTGGTTCCTGGTCAGAGT
TCAGCACCCAACCTGAGAACTTCTTGAAAGGCTGTAAGTGGGCTCCTGACGGTTCCTGCATCTTGACCAA
TAGTGCTGATAACATCTTGCGAATTTATAACCTGCCCCCAGAGCTGTACCATGAGGGGGAGCAGGTGGAA
TATGCAGAAATGGTCCCTGTCCTTCGAATGGTGGAAGGTGATACCATCTATGATTACTGCTGGTATTCTC
TGATGTCCTCAGCCCAGCCAGACACCTCCTACGTGGCCAGCAGCAGCCGGGAGAACCCGATTCATATCTG
GGACGCATTCACTGGAGAGCTCCGGGCTTCCTTTCGCGCCTACAACCACCTGGATGAGCTGACGGCAGCC
CATTCGCTCTGCTTCTCCCCGGATGGCTCCCAGCTCTTCTGTGGCTTCAACCGGACTGTGCGTGTTTTTT
CCACGGCCCGGCCTGGCCGAGACTGCGAGGTCCGAGCCACATTTGCAAAAAAGCAGGGCCAGAGCGGCAT
CATCTCCTGCATAGCCTTCAGCCCAGCCCAGCCCCTCTATGCCTGTGGCTCCTACGGCCGCTCCCTGGGT
CTGTATGCCTGGGATGATGGCTCCCCTCTCGCCTTGCTGGGAGGGCACCAAGGGGGCATCACCCACCTCT
GCTTTCATCCCGATGGCAACCGCTTCTTCTCAGGAGCCCGCAAGGATGCTGAGCTCCTGTGCTGGGATCT
CCGGCAGTCTGGTTACCCACTGTGGTCCCTGGGTCGAGAGGTGACCACCAATCAGCGCATCTACTTCGAT
CTGGACCCGACCGGGCAGTTCCTAGTGAGTGGCAGCACGAGCGGGGCTGTCTCTGTGTGGGACACGGACG
GGCCTGGCAATGATGGGAAGCCGGAGCCCGTGTTGAGTTTTCTGCCCCAGAAGGACTGCACCAATGGCGT
GAGCCTGCACCCTAGCCTGCCTCTCCTGGCCACTGCCTCCGGTCAGCGTGTGTTTCCTGAGCCCACAGAG
AGTGGGGACGAAGGAGAGGAGCTGGGCCTTCCCTTGCTCTCCACGCGCCACGTCCACCTTGAATGTCGGC
TTCAGCTCTGGTGGTGTGGGGGGGCGCCAGACTCCAGCATCCCTGATGATCACCAGGGCGAGAAAGGGCA
GGGAGGAACGGAGGGAGGTGTGGGTGAGCTGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC227773 representing NM_001143991
Red=Cloning site Green=Tags(s)

MKTLETQPLAPDCCPSDQDPAPAHPSPHASPMNKNADSELMPPPPERGDPPRLSPDPVAGSAVSQELREG
DPVSLSTPLETEFGSPSELSPRIEEQELSENTSLPAEEANGSLSEEEANGPELGSGKAMEDTSGEPAAED
EGDTAWNYSFSQLPRFLSGSWSEFSTQPENFLKGCKWAPDGSCILTNSADNILRIYNLPPELYHEGEQVE
YAEMVPVLRMVEGDTIYDYCWYSLMSSAQPDTSYVASSSRENPIHIWDAFTGELRASFRAYNHLDELTAA
HSLCFSPDGSQLFCGFNRTVRVFSTARPGRDCEVRATFAKKQGQSGIISCIAFSPAQPLYACGSYGRSLG
LYAWDDGSPLALLGGHQGGITHLCFHPDGNRFFSGARKDAELLCWDLRQSGYPLWSLGREVTTNQRIYFD
LDPTGQFLVSGSTSGAVSVWDTDGPGNDGKPEPVLSFLPQKDCTNGVSLHPSLPLLATASGQRVFPEPTE
SGDEGEELGLPLLSTRHVHLECRLQLWWCGGAPDSSIPDDHQGEKGQGGTEGGVGELI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001143991
ORF Size 1644 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001143991.2
RefSeq Size 1847 bp
RefSeq ORF 1647 bp
Locus ID 55135
UniProt ID Q9BUR4
Cytogenetics 17p13.1
MW 59.3 kDa
Summary This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. provided by RefSeq, Mar 2011
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Documents
Resources
"NM_001143991" in other vectors (4)
SKU Description Size Price
RC227773L3 Lenti-ORF clone of WRAP53 (Myc-DDK-tagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3 10 ug
$810.00
RC227773L4 Lenti-ORF clone of WRAP53 (mGFP-tagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3 10 ug
$810.00
RG227773 WRAP53 (tGFP-tagged) - Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3 10 ug
$710.00
SC325187 WRAP53 (untagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 3 10 ug
$562.00

Plasmid Map for RC227773

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC227773
Circular map for RC227773

file-alt Citations

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