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SQSTM1 (NM_001142298) Human Over-expression Lysate

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SKU
LY428020
Transient overexpression lysate of sequestosome 1 (SQSTM1), transcript variant 2 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC227337
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001142298, NP_001135770
Synonyms A170; DMRV; FTDALS3; NADGP; OSIL; p60; p62; p62B; PDB3; ZIP3
Predicted MW 47.69 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001135770
Locus ID 8878
Cytogenetics 5q35.3
Protein Families WB
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Other "SQSTM1" proteins (5)
SKU Description Size Price
PH303214 SQSTM1 MS Standard C13 and N15-labeled recombinant protein (NP_003891) 10 ug
$3,360.00
LC401285 Lyophilized SQSTM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428020 Lyophilized SQSTM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401285 Transient overexpression lysate of sequestosome 1 (SQSTM1), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP303214 Recombinant protein of human sequestosome 1 (SQSTM1), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Macroautophagy Regulates Nuclear NOTCH1 Activity Through Multiple p62 Binding Sites
Zhang, Guo, Wang et al
IUBMB Life (2018) 70 (10), 985-994 DOI: 10.1002/iub.1891
AI Summary: OriGene contributed to this study by providing siRNAs targeting ATG3, ATG5, ATG7, ATG16L1, and p62, which were used to investigate the roles of these genes in autophagy-mediated regulation of nuclear NOTCH1 activity.
Snippet: …SiRNAs against ATG3, ATG5, ATG7, ATG16L1, and p62 were purchased from Origene (three distinct siRNA sequences for each gene). …SiRNAs against ATG3, ATG5, ATG7, ATG16L1, and p62 were purchased from Origene (three distinct siRNA sequences for each gene). …
FigureFigure
Synergistic effects of HMG-CoA reductase inhibitor and angiotensin II receptor blocker on load-induced heart failure
Ito, Maejima, Tamura et al
FEBS Open Bio (2018) 8 (5), 799-816 DOI: 10.1002/2211-5463.12416
Cited by 7
AI Summary: In this study, OriGene’s mouse AT1R knockout (AT1R-/-) model (B6.129P2-Agtr1atm1Unc/J) was used to investigate the role of angiotensin II type 1 receptor in the cardiac effects of combined statin and ARB therapy in heart failure.
FigureFigureFigure
Drp1-Dependent Mitochondrial Autophagy Plays a Protective Role Against Pressure-Overload-Induced Mitochondrial Dysfunction and Heart Failure
Shirakabe, Zhai, Ikeda et al
Circulation (2016)
Cited by 1
AI Summary: In this study, the OriGene p62 antibody (TA307334) was used to assess autophagy-related protein levels, contributing to the evaluation of mitochondrial autophagy dynamics in heart tissue under pressure overload.
Snippet: Transduction, 611112), phospho-Drp1 (Ser616) (Cell Signaling, 4494S), phospho-Drp1 (Ser637) (Cell Signaling, 4867S), LC3 (BML, M186-3), p62 (ORIGENE, TA307334), Cox I (abcam, ab110413), Beclin 1 (Cell signaling, 3738S), PGC-1α (Santa Cruz, sc-13067), ... Cited by 1 Cite SaveSaving
Showing 1-3 of 3 papers.
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