OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

SQSTM1 (NM_001142298) Human Over-expression Lysate

Be the first to review this product
SKU
LC428020
Lyophilized SQSTM1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA502127 SQSTM1 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "SQSTM1 or NM_001142298"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC227337
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001142298, NP_001135770
Synonyms A170; DMRV; FTDALS3; NADGP; OSIL; p60; p62; p62B; PDB3; ZIP3
Predicted MW 47.69 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001135770
Locus ID 8878
Cytogenetics 5q35.3
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "SQSTM1" proteins (5)
SKU Description Size Price
PH303214 SQSTM1 MS Standard C13 and N15-labeled recombinant protein (NP_003891) 10 ug
$3,360.00
LC401285 Lyophilized SQSTM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401285 Transient overexpression lysate of sequestosome 1 (SQSTM1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY428020 Transient overexpression lysate of sequestosome 1 (SQSTM1), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP303214 Recombinant protein of human sequestosome 1 (SQSTM1), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Macroautophagy Regulates Nuclear NOTCH1 Activity Through Multiple p62 Binding Sites
Zhang, Guo, Wang et al
IUBMB Life (2018) 70 (10), 985-994 DOI: 10.1002/iub.1891
AI Summary: OriGene contributed to this study by providing siRNAs targeting ATG3, ATG5, ATG7, ATG16L1, and p62, which were used to investigate the roles of these genes in autophagy-mediated regulation of nuclear NOTCH1 activity.
Snippet: …SiRNAs against ATG3, ATG5, ATG7, ATG16L1, and p62 were purchased from Origene (three distinct siRNA sequences for each gene). …SiRNAs against ATG3, ATG5, ATG7, ATG16L1, and p62 were purchased from Origene (three distinct siRNA sequences for each gene). …
FigureFigure
Synergistic effects of HMG-CoA reductase inhibitor and angiotensin II receptor blocker on load-induced heart failure
Ito, Maejima, Tamura et al
FEBS Open Bio (2018) 8 (5), 799-816 DOI: 10.1002/2211-5463.12416
Cited by 7
AI Summary: In this study, OriGene’s mouse AT1R knockout (AT1R-/-) model (B6.129P2-Agtr1atm1Unc/J) was used to investigate the role of angiotensin II type 1 receptor in the cardiac effects of combined statin and ARB therapy in heart failure.
FigureFigureFigure
Drp1-Dependent Mitochondrial Autophagy Plays a Protective Role Against Pressure-Overload-Induced Mitochondrial Dysfunction and Heart Failure
Shirakabe, Zhai, Ikeda et al
Circulation (2016)
Cited by 1
AI Summary: In this study, the OriGene p62 antibody (TA307334) was used to assess autophagy-related protein levels, contributing to the evaluation of mitochondrial autophagy dynamics in heart tissue under pressure overload.
Snippet: Transduction, 611112), phospho-Drp1 (Ser616) (Cell Signaling, 4494S), phospho-Drp1 (Ser637) (Cell Signaling, 4867S), LC3 (BML, M186-3), p62 (ORIGENE, TA307334), Cox I (abcam, ab110413), Beclin 1 (Cell signaling, 3738S), PGC-1α (Santa Cruz, sc-13067), ... Cited by 1 Cite SaveSaving
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products