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DNMT1 (NM_001130823) Human Over-expression Lysate

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SKU
LY427274
Transient overexpression lysate of DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC226414
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001130823, NP_001124295
Synonyms ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Predicted MW 184.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001124295
Locus ID 1786
Cytogenetics 19p13.2
Protein Families WB
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
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Other "DNMT1" proteins (3)
SKU Description Size Price
PH326414 DNMT1 MS Standard C13 and N15-labeled recombinant protein (NP_001124295) 10 ug
$3,360.00
LC427274 Lyophilized DNMT1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP326414 Recombinant protein of human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Targeting EP4 downstream c-Jun through ERK1/2-mediated reduction of DNMT1 reveals novel mechanism of solamargine-inhibited growth of lung cancer cells
Chen, Tang, Xiao et al
J. Cell. Mol. Med. (2016)
Cited by 40
AI Summary: OriGene products, specifically the pCMV6-AC-DNMT1, pCMV6-AC-c-Jun, and pCMV6-AC-EP4 overexpression constructs, were used in this study to investigate the roles of DNMT1, c-Jun, and EP4 in solamargine’s inhibition of lung cancer cell growth.
Snippet: Transient transfection assay. This procedure was reported previously [12]. The control or EP4, DNMT1 and c-Jun overexpression constructs (pCMV6-AC-DNMT1, pCMV6-AC-c-Jun, pCMV6-AC-EP4) were obtained from OriGene Technologies, Inc. (Rockville, ... Cite SaveSaving
Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I
Li, Wu, Zheng et al
J. Exp. Clin. Cancer Res. (2016) 35 (1), 112 DOI: 10.1186/s13046-016-0388-x
Cited by 49
AI Summary: OriGene products, specifically the Myc-DDK-tagged or Myc/FLAG-tagged human DNMT1 and EZH2 expression constructs, were used in this study to enable exogenous overexpression of DNMT1 and EZH2, which helped elucidate their roles in the mechanism by which polyphyllin I inhibits human lung cancer cells.
Snippet: sample. In the separated experiment, 2 μg of the control (pCMV6) and expression constructs containing Myc-DDK-tagged- or Myc/FLAG-tagged ORFs of human DNMT1 or EZH2 obtained from OriGene Technologies, Inc. (Rockville ... Cite SaveSaving
FigureFigureFigure
Showing 1-2 of 2 papers.
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