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DNMT1 (NM_001130823) Human Over-expression Lysate

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SKU
LC427274
Lyophilized DNMT1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC226414
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001130823, NP_001124295
Synonyms ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Predicted MW 184.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001124295
Locus ID 1786
Cytogenetics 19p13.2
Protein Families WB
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
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Other "DNMT1" proteins (3)
SKU Description Size Price
PH326414 DNMT1 MS Standard C13 and N15-labeled recombinant protein (NP_001124295) 10 ug
$3,360.00
LY427274 Transient overexpression lysate of DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP326414 Recombinant protein of human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Targeting EP4 downstream c-Jun through ERK1/2-mediated reduction of DNMT1 reveals novel mechanism of solamargine-inhibited growth of lung cancer cells
Chen, Tang, Xiao et al
J. Cell. Mol. Med. (2016)
Cited by 40
AI Summary: OriGene products, specifically the pCMV6-AC-DNMT1, pCMV6-AC-c-Jun, and pCMV6-AC-EP4 overexpression constructs, were used in this study to investigate the roles of DNMT1, c-Jun, and EP4 in solamargine’s inhibition of lung cancer cell growth.
Snippet: Transient transfection assay. This procedure was reported previously [12]. The control or EP4, DNMT1 and c-Jun overexpression constructs (pCMV6-AC-DNMT1, pCMV6-AC-c-Jun, pCMV6-AC-EP4) were obtained from OriGene Technologies, Inc. (Rockville, ... Cite SaveSaving
Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I
Li, Wu, Zheng et al
J. Exp. Clin. Cancer Res. (2016) 35 (1), 112 DOI: 10.1186/s13046-016-0388-x
Cited by 49
AI Summary: OriGene products, specifically the Myc-DDK-tagged or Myc/FLAG-tagged human DNMT1 and EZH2 expression constructs, were used in this study to enable exogenous overexpression of DNMT1 and EZH2, which helped elucidate their roles in the mechanism by which polyphyllin I inhibits human lung cancer cells.
Snippet: sample. In the separated experiment, 2 μg of the control (pCMV6) and expression constructs containing Myc-DDK-tagged- or Myc/FLAG-tagged ORFs of human DNMT1 or EZH2 obtained from OriGene Technologies, Inc. (Rockville ... Cite SaveSaving
FigureFigureFigure
Showing 1-2 of 2 papers.
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