DNMT1 (NM_001130823) Human Recombinant Protein
SKU
TP326414
Recombinant protein of human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1, 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 184.6 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | DNMT1 activity verified in a biochemical assay: DNMT1 (DNA (cytosine-5-)-methyltransferase 1) (TP326414) is a key methyltransferase that is responsible for maintaining methylation patterns established in development. DNMT1 preferentially methylates hemi-methylated CpG di-nucleotides and associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. Varying concentrations of DNMT1 were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured. |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_001124295 |
| Locus ID | 1786 |
| UniProt ID | P26358 |
| Cytogenetics | 19p13.2 |
| RefSeq ORF | 4896 |
| Synonyms | ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT |
| Summary | This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2016 |
| Protein Categories | Intracellular Proteins, Nervous system Diseases, Transciption Factors |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "DNMT1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH326414 | DNMT1 MS Standard C13 and N15-labeled recombinant protein (NP_001124295) | 10 ug |
$3,360.00
|
|
| LC427274 | Lyophilized DNMT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY427274 | Transient overexpression lysate of DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
Citations
WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage
Han, Wen, García-Rubio et al
Nat Commun (2022) 13 (1), 3743 DOI: 10.1038/s41467-022-31415-z
Han, Wen, García-Rubio et al
Nat Commun (2022) 13 (1), 3743 DOI: 10.1038/s41467-022-31415-z
Cited by 37
AI Summary: OriGene products, specifically RPA1 (TP302066), RPA2 (TP305715), MAX (TP320343), ARP3 (TP308460), FANCA (TP761743), XRCC2 (TP308330), DNMT1 (TP326414), MYC (TP301611), NF-κB1 (TP308384), and STAT1 (TP313858), were used as key reagents to investigate the molecular mechanisms by which WASp modulates RPA function and maintains genomic stability under replication stress and DNA damage.
Snippet: ns RPA1 (TP302066), RPA2 (TP305715), MAX (TP320343), ARP3 (TP308460), FANCA (TP761743), XRCC2 (TP308330), DNMT1 (TP326414), MYC (TP301611), NF-κB1 (TP308384) and STAT1 (TP313858) were purchased from OriGene (Rockville, MD). ARP2 (ab217837) was purchased from Abcam (Cambridge, MA). Heterotrimeric RPA and RPA1 mutant forms of RPA were expressed as recombinant protein E. coli and them purified over
DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro
Cree, Fredericks, Miller et al
FEBS Lett. (2016)
Cree, Fredericks, Miller et al
FEBS Lett. (2016)
Cited by 57
AI Summary: OriGene contributed to this study by providing purified DNA methyltransferase proteins, which were used to investigate the interaction between DNMTs and DNA G-quadruplexes in vitro.
Snippet: Measurement data were collected for an average of three scans over a range of 220–340 nm at 25 °C and baseline corrected (buffer only). Proteins. Purified DNA methyltransferase proteins were purchased from OriGene (Rockville, MD, USA). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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