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NDRG4 (NM_001130487) Human Over-expression Lysate

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SKU
LY427222
Transient overexpression lysate of NDRG family member 4 (NDRG4), transcript variant 2 (as WB positive control)
  $436.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC225600
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001130487, NP_001123959
Synonyms BDM1; SMAP-8; SMAP8
Predicted MW 42.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001123959
Locus ID 65009
Cytogenetics 16q21
Protein Families WB
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Other "NDRG4" proteins (10)
SKU Description Size Price
PH321058 NDRG4 MS Standard C13 and N15-labeled recombinant protein (NP_065198) 10 ug
$3,360.00
PH321301 NDRG4 MS Standard C13 and N15-labeled recombinant protein (NP_075061) 10 ug
$3,360.00
LC402788 Lyophilized NDRG4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC411459 Lyophilized NDRG4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427222 Lyophilized NDRG4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402788 Transient overexpression lysate of NDRG family member 4 (NDRG4), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY411459 Transient overexpression lysate of NDRG family member 4 (NDRG4), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP321058 Recombinant protein of human NDRG family member 4 (NDRG4), transcript variant 1, 20 µg 20 ug
$867.00
TP321301 Recombinant protein of human NDRG family member 4 (NDRG4), transcript variant 3, 20 µg 20 ug
$867.00
TP761034 Purified recombinant protein of Human NDRG family member 4 (NDRG4), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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