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G protein alpha S (GNAS) (NM_000516) Human Over-expression Lysate

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SKU
LY424674
Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214197
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000516, NP_000507
Synonyms AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; PITA3; POH; SCG6; SgVI
Predicted MW 45.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000507
Locus ID 2778
Cytogenetics 20q13.32
Protein Families WB
Protein Pathways Calcium signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term depression, Melanogenesis, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection
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Other "G protein alpha S" proteins (15)
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LC402577 Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC421439 Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC421440 Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC424674 Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY402577 Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 4 (as WB positive control) 100 ug
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LY409171 Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 3 (as WB positive control) 100 ug
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LY421439 Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 6 (as WB positive control) 100 ug
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LY421440 Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 7 (as WB positive control) 100 ug
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TP314197 Recombinant protein of human GNAS complex locus (GNAS), transcript variant 1, 20 µg 20 ug
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TP314318 Recombinant protein of human GNAS complex locus (GNAS), transcript variant 3, 20 µg 20 ug
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TP316883 Purified recombinant protein of Homo sapiens GNAS complex locus (GNAS), transcript variant 7, 20 µg 20 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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