G protein alpha S (GNAS) (NM_080426) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 44.1 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_536351 |
| Locus ID | 2778 |
| UniProt ID | P63092 |
| Cytogenetics | 20q13.32 |
| RefSeq Size | 1551 |
| RefSeq ORF | 1142 |
| Synonyms | AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; PITA3; POH; SCG6; SgVI |
| Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. provided by RefSeq, Aug 2012 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Calcium signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term depression, Melanogenesis, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "G protein alpha S" proteins (15)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH314197 | GNAS MS Standard C13 and N15-labeled recombinant protein (NP_000507) | 10 ug |
$3,360.00
|
|
| PH314318 | GNAS MS Standard C13 and N15-labeled recombinant protein (NP_536351) | 10 ug |
$3,360.00
|
|
| PH316883 | GNAS MS Standard C13 and N15-labeled recombinant protein (NP_001070957) | 10 ug |
$3,360.00
|
|
| LC402577 | Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC409171 | Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC421439 | Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC421440 | Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC424674 | Lyophilized GNAS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY402577 | Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 4 (as WB positive control) | 100 ug |
$436.00
|
|
| LY409171 | Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| LY421439 | Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 6 (as WB positive control) | 100 ug |
$436.00
|
|
| LY421440 | Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 7 (as WB positive control) | 100 ug |
$436.00
|
|
| LY424674 | Transient overexpression lysate of GNAS complex locus (GNAS), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| TP314197 | Recombinant protein of human GNAS complex locus (GNAS), transcript variant 1, 20 µg | 20 ug |
$737.00
|
|
| TP316883 | Purified recombinant protein of Homo sapiens GNAS complex locus (GNAS), transcript variant 7, 20 µg | 20 ug |
$737.00
|
|
Citations
ADCK2 Haploinsufficiency Reduces Mitochondrial Lipid Oxidation and Causes Myopathy Associated with CoQ Deficiency
Vázquez-Fonseca, Schaefer, Navas-Enamorado et al
J Clin Med (2019) 8 (9) DOI: 10.3390/jcm8091374
Vázquez-Fonseca, Schaefer, Navas-Enamorado et al
J Clin Med (2019) 8 (9) DOI: 10.3390/jcm8091374
AI Summary: OriGene’s recombinant human Gαs protein (TP314318) was used in the reaction mix to investigate mitochondrial function and signaling pathways in the study of ADCK2 haploinsufficiency and its effects on mitochondrial lipid oxidation and myopathy.
Snippet: The reaction mix contains 500 nM recombinant human Gαs (Origene TP314318;Rockville, MD), nonmyristoylated rat Gαi proteins (gift of Dr. Carmen Dessauer and Dr. Tanya Baldwin) or recombinant human GAPDH (Abcam ab77109),
DHHC5 Mediates β-Adrenergic Signaling in Cardiomyocytes by Targeting Gα Proteins
Chen, Marsden, Scott et al
Biophys. J. (2019) 118 (4), 826-835 DOI: 10.1016/j.bpj.2019.08.018
Chen, Marsden, Scott et al
Biophys. J. (2019) 118 (4), 826-835 DOI: 10.1016/j.bpj.2019.08.018
AI Summary: OriGene’s recombinant human Gαs protein (TP314318) was used in palmitoylation assays to demonstrate that Gαs is a direct substrate of DHHC5, supporting the study’s investigation into β-adrenergic signaling in cardiomyocytes.
Snippet: The reaction mix contains 500 nM recombinant human Gαs (Origene TP314318;Rockville, MD), nonmyristoylated rat Gαi proteins (gift of Dr. Carmen Dessauer and Dr. Tanya Baldwin) or recombinant human GAPDH (Abcam ab77109), 200 nM dithiothreitol, 300 μM
DHHC5 mediates beta-adrenergic signaling in cardiomyocytes by targeting Gα proteins
Chen, Marsden, Scott et al
Biophysical Journal (2019)
Chen, Marsden, Scott et al
Biophysical Journal (2019)
AI Summary: OriGene’s recombinant human Gαs protein (TP314318) was used in palmitoylation assays to investigate the direct modification of Gα proteins by DHHC5 in the study.
Snippet: The reaction mix contains 500nM recombinant human Gαs (Origene TP314318), non- myristoylated rat Gαi proteins (Gift of Dr. Carmen Dessauer and Dr. Tanya Baldwin) or recombinant human GAPDH (abcam ab77109), 200nM DTT, 300µM DDM, and 5µM palmitoyl
DHHC5 REGULATES BETA-ADRENERGIC SIGNALING IN THE HEART THROUGH PALMITOYLATION OF G ALPHA PROTEINS
Chen
Thesis (2019)
Chen
Thesis (2019)
Snippet: Rabbit monoclonal anti-FLAG (CST147935) or RFP-Trap (ChromoTek) were used to immunoprecipitate DHHS/C5. The reaction mix contains 500 nM recombinant human Gαs (Origene TP314318), non-myristoylated rat Gαi proteins (Gift of Dr. Carmen Dessauer and Dr. Tanya Baldwin) or recombinant human GAPDH (abcam ab77109), 200 nM DTT, 300 µM DDM, and 5 µM palmitoyl CoA{N-[(7- nitro-2-1,3- benzoxadiazol-4-yl)-methyl]amino} (Avanti lipids)
DHHC5 Mediates b-Adrenergic Signaling in Cardiomyocytes by Targeting Ga Proteins
Chen, Marsden, Scott et al
Biophysical Journal (2019) 117, 1–10 DOI: 10.1016/j.bpj.2019.08.018/attachment/8cbd88b5-b078-4bff-9fa8-4d7bb971b18d/mmc4
Chen, Marsden, Scott et al
Biophysical Journal (2019) 117, 1–10 DOI: 10.1016/j.bpj.2019.08.018/attachment/8cbd88b5-b078-4bff-9fa8-4d7bb971b18d/mmc4
AI Summary: OriGene’s recombinant human Gas protein (TP314318) was used in this study to investigate the role of DHHC5 in β-adrenergic signaling in cardiomyocytes.
Snippet: The reaction mix contains 500 nM recombinant human Gas (Origene TP314318;Rockville, MD), nonmyristoylated rat Gai proteins (gift of Dr. Carmen Dessauer and Dr. Tanya Baldwin) or recombinant human GAPDH (Abcam ab77109), 200 nM dithiothreitol, 300 mM
Showing 1-5 of 5 papers.
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