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NT5C3 (NT5C3A) (NM_001002010) Human Over-expression Lysate

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SKU
LY424319
Transient overexpression lysate of 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 1 (as WB positive control)
  • LY424319 is the same product as LY425069.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222818
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001002010, NP_001002010
Synonyms cN-III; hUMP1; NT5C3; P5'N-1; P5N-1; p36; PN-I; POMP; PSN1; UMPH; UMPH1
Predicted MW 37.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001002010
Locus ID 51251
Cytogenetics 7p14.3
Protein Families WB
Protein Pathways Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
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Other "NT5C3" proteins (9)
SKU Description Size Price
PH305629 NT5C3 MS Standard C13 and N15-labeled recombinant protein (NP_057573) 10 ug
$3,360.00
PH309741 NT5C3 MS Standard C13 and N15-labeled recombinant protein (NP_001002009) 10 ug
$3,360.00
LC413931 Lyophilized NT5C3A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424318 Lyophilized NT5C3A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424319 Lyophilized NT5C3A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY413931 Transient overexpression lysate of 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY424318 Transient overexpression lysate of 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP305629 Recombinant protein of human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 3, 20 µg 20 ug
$737.00
TP309741 Recombinant protein of human 5'-nucleotidase, cytosolic III (NT5C3), transcript variant 2, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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