AKT1 (NM_001014432) Human Over-expression Lysate

CAT#: LY423057

Transient overexpression lysate of v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2

Product images

Western validation with an anti-DDK antibody; L: Control HEK293 lysate R: Over-expression lysate

Specifications

Product Data

• Description: Transient overexpression lysate of v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2
• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC220361
• Tag: C-Myc/DDK
• Detection Antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• Accession Number: NM_001014432, NP_001014432
• Other Names: AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
• Predicted MW: 55.7 kDa
• Components: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20°C. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
• Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20°C before shipping.

Reference Data

• RefSeq: NP_001014432
• Locus ID: 207
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway