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AKT1 (NM_001014431) Human Over-expression Lysate

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SKU
LY423056
Transient overexpression lysate of v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201850
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001014431, NP_001014431
Synonyms AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Predicted MW 55.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001014431
Locus ID 207
Cytogenetics 14q32.33
Protein Families WB
Protein Pathways Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway
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Other "AKT1" proteins (15)
SKU Description Size Price
PH301850 AKT1 MS Standard C13 and N15-labeled recombinant protein (NP_001014431) 10 ug
$3,360.00
PH320257 AKT1 MS Standard C13 and N15-labeled recombinant protein (NP_005154) 10 ug
$3,360.00
PH320361 AKT1 MS Standard C13 and N15-labeled recombinant protein (NP_001014432) 10 ug
$3,360.00
LC401580 Lyophilized AKT1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423056 Lyophilized AKT1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423057 Lyophilized AKT1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC425358 Lyophilized AKT1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY401580 Transient overexpression lysate of v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY423057 Transient overexpression lysate of v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY425358 Transient overexpression lysate of v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP301850 Recombinant protein of human v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3, 20 µg 20 ug
$737.00
TP320257 Recombinant protein of human v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 1, 20 µg 20 ug
$737.00
TP320361 Recombinant protein of human v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 2, 20 µg 20 ug
$737.00
TP700055 Recombinant protein of human v-akt murine thymoma viral oncogene homolog 1 (AKT1) mutant (E17K), transcript variant 1 20 ug
$900.00
TP700060 Recombinant protein of human v-akt murine thymoma viral oncogene homolog 1 (AKT1 mutant S473D), transcript variant 1, with C-terminal MYC/DDK tag, expressed in human cells, 20ug 20 ug
$900.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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