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CTLA4 (NM_001037631) Human Over-expression Lysate

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SKU
LY421970
Transient overexpression lysate of cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 2 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213631
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001037631, NP_001032720
Synonyms ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12
Predicted MW 19.15 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001032720
Locus ID 1493
Cytogenetics 2q33.2
Protein Families WB
Protein Pathways Autoimmune thyroid disease, Cell adhesion molecules (CAMs), T cell receptor signaling pathway
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Other "CTLA4" proteins (9)
SKU Description Size Price
LC417438 Lyophilized CTLA4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421970 Lyophilized CTLA4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417438 Transient overexpression lysate of cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP700216 Purified recombinant protein of Human cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells 20 ug
$900.00
TP700217 Purified recombinant protein of Human cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1, with C-terminal human Fc and his tags, expressed in human cells 20 ug
$900.00
TP721230 Purified recombinant protein of Human cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1 10 ug
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TP723874 Purified recombinant protein of Human cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1 10 ug
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TP723940 Recombinant human CTLA-4 protein with C-terminal mouse Fc and 6×His tag 100 ug
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TP724014 Recombinant human CTLA-4 protein with C-terminal human Fc tag 100 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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